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Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L

Author

Listed:
  • J.I. Flores Solís

    (Mendel University of Agriculture and Forestry in Brno, Czech Republic)

  • P. Mlejnek

    (Mendel University of Agriculture and Forestry in Brno, Czech Republic)

  • K. Studená

    (Mendel University of Agriculture and Forestry in Brno, Czech Republic)

  • S. Procházka

    (Mendel University of Agriculture and Forestry in Brno, Czech Republic)

Abstract

Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient expression of GUS gene coding for b-glucuronidase in Chenopodium rubrum. Further the results indicated that the age of seedlings is one of the limiting factors affecting the potency of Agrobacterium tumefaciens infection. Histochemical detection of b-glucuronidase activity revealed that two-days-old seedlings were much more susceptible to infection than ten-days-old ones. According to our results SAAT technology could provide an efficient tool for obtaining stable transformants when applied to two-days-old seedlings of Chenopodium rubrum.

Suggested Citation

  • J.I. Flores Solís & P. Mlejnek & K. Studená & S. Procházka, 2003. "Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 49(6), pages 255-260.
    • Handle: RePEc:caa:jnlpse:v:49:y:2003:i:6:id:4122-pse
    DOI: 10.17221/4122-PSE
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