IDEAS home Printed from https://ideas.repec.org/a/caa/jnlpse/v48y2002i11id4400-pse.html
   My bibliography  Save this article

Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

Author

Listed:
  • P. Vejl

    (Czech University of Agriculture in Prague, Czech Republic)

  • S. Skupinová

    (Czech University of Agriculture in Prague, Czech Republic)

  • P. Sedlák

    (Czech University of Agriculture in Prague, Czech Republic)

  • J. Domkářová

    (Czech University of Agriculture in Prague, Czech Republic)

Abstract

Progressive methods of molecular analyses of DNA are routinely used in the fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described in this article. European populations of PCN - Šluknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown pathotype from Kašperské hory (K) locality were used. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1-Rro1 was product 411 bp detected (only in cause G. rostochiensis), by using of set Fpa2-Rpa1 the product 239 bp was detected (only G. pallida). For these reasons the identity of the European populations was confirmed. Cysts of population K were identified as G. pallida.

Suggested Citation

  • P. Vejl & S. Skupinová & P. Sedlák & J. Domkářová, 2002. "Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 48(11), pages 486-489.
    • Handle: RePEc:caa:jnlpse:v:48:y:2002:i:11:id:4400-pse
    DOI: 10.17221/4400-PSE
    as

    Download full text from publisher

    File URL: http://pse.agriculturejournals.cz/doi/10.17221/4400-PSE.html
    Download Restriction: free of charge
    File URL: http://pse.agriculturejournals.cz/doi/10.17221/4400-PSE.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/4400-PSE?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. P. Sedlák & M. Melounová & S. Skupinová & P. Vejl & J. Domkářová, 2004. "Study of European and Czech populations of potato cyst nematodes (Globodera rostochiensis and G. pallida) by RAPD method," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 50(2), pages 70-74.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlpse:v:48:y:2002:i:11:id:4400-pse. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.