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Analysis of chromosome termini in potato varieties

Author

Listed:
  • J. Fajkus

    (Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic)

  • M. Novotná

    (Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic)

  • J. Ptáček

    (Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic)

Abstract

Chromosomes of potato (Solanum tuberosum) are terminated by telomeres, which are formed by tandemly repeated [TTTAGGG]n oligonucleotide sequence. The total length of blocks of telomeric DNA has been known to vary largely among plant species and their varieties, and also among individual chromosome arms within a single nucleus. To check for such differences in potato varieties, which could be of a possible use in genotyping, we performed pulsed-field gel electrophoretic analysis of terminal restriction fragments in selected potato varieties. We found a typical range of telomere lengths of 20-60 kb in most analysed varieties. In one of analysed varieties (Monalisa), telomeres of wider span (up to 80 kb) have been observed. Most of restriction enzymes (PvuII, HaeIII, TaqI) produced a resulting smeared hybridisation pattern of telomeres. When using BglII, however, a doublet hybridisation band could be observed. This may reflect differences in composition of telomere-associated sequences at different chromosome ends.

Suggested Citation

  • J. Fajkus & M. Novotná & J. Ptáček, 2002. "Analysis of chromosome termini in potato varieties," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 48(11), pages 477-479.
    • Handle: RePEc:caa:jnlpse:v:48:y:2002:i:11:id:4398-pse
    DOI: 10.17221/4398-PSE
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