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Molecular diagnosis of red rot of sugarcane in Pakistan and in vitro control of isolated pathogen using rhizobacteria

Author

Listed:
  • Zimen Ahmad

    (Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)

  • Muhammad Asad Farooq

    (Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan)

  • Amama Fatima

    (Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan)

  • Syeda Gul Nisa

    (Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)

  • Sania Tabassum

    (Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan)

  • Bushra Ismat

    (Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan)

  • Sundus Waseem

    (Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan)

  • Umer Iqbal

    (Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan)

  • Asma Akbar

    (Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan)

  • Urooj Haroon

    (Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)

  • Hassan Javed Chaudhary

    (Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)

  • Tauseef Tabassum

    (Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan)

  • Ahmed Z Dewidar

    (Prince Sultan Institute of Environmental, Water and Desert Research, King Saud University, Riyadh, Saudi Arabia)

  • Abdullah Alaklabi

    (Department of Biology, Faculty of Science, University of Bisha, Bisha, Saudi Arabia)

  • Muhammad Farooq Hussain Munis

    (Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)

Abstract

Sugarcane (Saccharum officinarum L.) is a key cash crop, and its production is influenced by a variety of phytopathogens in different parts of the world. During consecutive field surveys, sugarcane stalks with red rot symptoms were observed in three provinces of Pakistan (Punjab, Sindh, and Khyber Pakhtunkhwa). Cane samples with visible symptoms were plated on Potato Dextrose Agar (PDA) media. Morphological and microscopic observations identified this pathogen as Colletotrichum falcatum. Comparative molecular study of amplified sequences of β-tubulin (Bt) and Actin (ACT) genes showed 100% similarity and ITS sequence showed 99% similarity with C. falcatum. For in vitro control of red rot, eight bacterial strains from the rhizosphere of sugarcane were isolated. These strains showed variable growth inhibition of C. falcatum in dual culture method. Among all tested strains, Bacillus amyloliquefaciens and B. altitudinis exhibited best antifungal activities. Based on these results, tested bacterial strains (B. amyloliquefaciens and B. altitudinis) can be recommended as effective biocontrol agents to manage red rot disease of sugarcane.

Suggested Citation

  • Zimen Ahmad & Muhammad Asad Farooq & Amama Fatima & Syeda Gul Nisa & Sania Tabassum & Bushra Ismat & Sundus Waseem & Umer Iqbal & Asma Akbar & Urooj Haroon & Hassan Javed Chaudhary & Tauseef Tabassum , 2023. "Molecular diagnosis of red rot of sugarcane in Pakistan and in vitro control of isolated pathogen using rhizobacteria," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 59(2), pages 124-133.
    • Handle: RePEc:caa:jnlpps:v:59:y:2023:i:2:id:45-2022-pps
    DOI: 10.17221/45/2022-PPS
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