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PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review

Author

Listed:
  • Seyed Mahyar Mirmajlessi

    (Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
    Department of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia)

  • Maria Jennifer Sjölund

    (Science and Advice for Scottish Agriculture (SASA), Edinburgh, UK)

  • Marika Mänd

    (Department of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia)

  • Marianne Loiseau

    (French Agency for Food, Environmental and Occupational Health and Safety, Angers, France)

  • Vincenza Ilardi

    (Centro Difesa e Certificazione, CREA, Rome, Italy)

  • Geert Haesaert

    (Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium)

  • Reet Karise

    (Department of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia)

  • Richard Alexander Gottsberger

    (Department for Molecular Diagnostics of Plant Diseases, Institute for Sustainable Plant Production, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria)

  • Jason Sumner-Kalkun

    (Science and Advice for Scottish Agriculture (SASA), Edinburgh, UK)

  • Assunta Bertaccini

    (Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy)

Abstract

'Candidatus Liberibacter solanacearum' is an economically important pathogen in the Americas, New Zealand and Europe. The primary objective of this review is to systematically investigate the polymerase chain reaction (PCR)-based methods used for its detection in plant samples. Several databases were searched from the inception of the relevant literature up to August 2018. This review identified 53 studies that met all the inclusion criteria. The performance of the different methods was also compared, however due to data heterogeneity and insufficient evidence on the sensitivity of all assays used, a meta-analysis of the data was not possible. Nonetheless, the review indicates that the rtPCR designed to the 16S ribosomal RNA gene can be routinely employed as a fast, cost-effective, and reliable detection technique in diagnostic laboratories.

Suggested Citation

  • Seyed Mahyar Mirmajlessi & Maria Jennifer Sjölund & Marika Mänd & Marianne Loiseau & Vincenza Ilardi & Geert Haesaert & Reet Karise & Richard Alexander Gottsberger & Jason Sumner-Kalkun & Assunta Bert, 2019. "PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 55(4), pages 229-242.
    • Handle: RePEc:caa:jnlpps:v:55:y:2019:i:4:id:145-2018-pps
    DOI: 10.17221/145/2018-PPS
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