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Real time PCR quantification of Sclerotium rolfsii in chilli tissue and soil

Author

Listed:
  • Yu-Han GAO

    (Key Laboratory of Pest Management in Crops of the Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China)

  • Wu MIAO

    (Xiangyan Seed Co., Changsha, Hunan, P.R. China)

  • Rong-Jun GUO

    (Key Laboratory of Pest Management in Crops of the Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China)

  • Shi-Dong LI

    (Key Laboratory of Pest Management in Crops of the Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China)

Abstract

Root rot disease caused by Sclerotium rolfsii is one of the important factors limiting chilli production in southern China. Rapid methods of detection and identification of S. rolfsii are required for disease prediction and management. Herein, we adapted a four-step real time quantitative PCR method to detect S. rolfsii using new designed primer pair S301S/S301A. By using this assay, S. rolfsii was detected in ten soil samples and eight plant samples at concentrations as low as 10-7 g mycelium/g soil or plant, both with and without typical symptoms, while tissue-culturing method could only detect the fungus in two samples. This rapid and quantitative method is efficient for detection and determination of S. rolfsii population in soil and plants.

Suggested Citation

  • Yu-Han GAO & Wu MIAO & Rong-Jun GUO & Shi-Dong LI, 2015. "Real time PCR quantification of Sclerotium rolfsii in chilli tissue and soil," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 51(2), pages 61-66.
    • Handle: RePEc:caa:jnlpps:v:51:y:2015:i:2:id:43-2014-pps
    DOI: 10.17221/43/2014-PPS
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