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Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR

Author

Listed:
  • Muthusamy Karthikeyan

    (Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, Tamil Nadu, India)

  • Krishnan Radhika

    (Department of Soil Science and Agricultural Chemistry, Annamalai University, Tamil Nadu, India)

  • Ramanujam Bhaskaran

    (Coconut Research Station, Tamil Nadu, India)

  • Subramanian Mathiyazhagan

    (Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, Tamil Nadu, India)

  • Ramasamy Samiyappan

    (Department of Soil Science and Agricultural Chemistry, Annamalai University, Tamil Nadu, India)

  • Rethinasamy Velazhahan

    (Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, Tamil Nadu, India)

Abstract

Molecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymerase chain reaction (PCR) tests, the primer generated from the internal transcribed spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR product of 167 bp in size, was used. Apparently healthy palms in two coconut gardens were tested for Ganoderma disease by ELISA test using basidiocarp protein antiserum. Field trials were laid out in these early-diagnosed palms for the management of the disease. Based on the ELISA results, Pseudomonas fluorescens + Trichoderma viride with chitin amended treatments arrested the multiplication of the pathogen and within 6 months showed an optical density (OD) below the level of infected plants. Integrated Disease Management (IDM) and fungicide tridemorph treated palms showed OD values below infection level within 7 months, and T. harzianum and P. fluorescens + T. viride treated palms showed OD values below infection level in 8 months.

Suggested Citation

  • Muthusamy Karthikeyan & Krishnan Radhika & Ramanujam Bhaskaran & Subramanian Mathiyazhagan & Ramasamy Samiyappan & Rethinasamy Velazhahan, 2006. "Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 42(2), pages 49-57.
    • Handle: RePEc:caa:jnlpps:v:42:y:2006:i:2:id:2771-pps
    DOI: 10.17221/2771-PPS
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