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Biological and serological procedures to detect three nepoviruses in fruit trees

Author

Listed:
  • Jaroslav Polák

    (Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

  • Michaela Chaloupková

    (Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

  • Milan Jokeš

    (Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

Abstract

Cherry leaf roll virus (CLRV), Myrobalan latent ringspot virus (MLRSV) and Strawberry latent ringspot virus (SLRSV) were transferred by budding to woody trees, hybrid Ishtara, peach cv. GF 305 and cv. Lesiberian. Three buffers with antioxidants and stabilisers: 0.01M phosphate with 1% caffeine; 0.007M phosphate-0.01M veronal with 0.01M cysteine hydrochloride and 0.007 EDTA; 0.015M phosphate with 1% nicotine and 0.066M phosphate buffer without additives were compared for their efficiency in mechanical transmission from woody sources to herbaceous hosts (Chenopodium quinoa and C. amaranticolor). 0.007M phosphate-0.01M veronal buffer with 0.01M cysteine hydrochloride, and 0.007 EDTA and 0.015M phosphate buffer with 1% nicotine were found to be the best buffers for the three nepoviruses. Both biological transmission to herbaceous assay hosts and detection by ELISA in the investigated tree are necessary to reliably detect the three nepoviruses. Biological detection is reliable from April to June, and in September and October. ELISA detection is also more difficult in July and August. The suitability of C. quinoa and C. amaranticolor to maintain CLRV, MLRSV and SLRSV was compared. C. amaranticolor plants were found to be more suitable for CLRV and SLRSV, infected plants grow over 6 months after mechanical inoculation by the nepoviruses. C. quinoa plants proved to be most suitable for maintenance of MLRSV, while C. amaranticolor is a symptomless host of MLRSV. Reinoculation with the nepoviruses is recommended in intervals of 4 to 6 months.

Suggested Citation

  • Jaroslav Polák & Michaela Chaloupková & Milan Jokeš, 2004. "Biological and serological procedures to detect three nepoviruses in fruit trees," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 40(4), pages 121-127.
    • Handle: RePEc:caa:jnlpps:v:40:y:2004:i:4:id:468-pps
    DOI: 10.17221/468-PPS
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    Citations

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    Cited by:

    1. Jaroslav POLÁK, 2008. "The production of antiserum against Myrobalan latent ringspot virus for detection of the virus using ELISA," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 44(1), pages 6-8.
    2. Shesh Kumari, 2009. "Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RT-PCR," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 45(4), pages 140-143.

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