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Characterisation of a PR-1: Luciferase transgenic line deployed to uncover novel defence-related Arabidopsis mutants by luciferase imaging

Author

Listed:
  • A. Chini

    (Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *)

  • S. Murray

    (Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *)

  • J. Grant

    (Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *)

  • C. Thomson

    (Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *)

  • G. Loake

    (Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *)

Abstract

In order to identify components of the defence signalling network that may contribute to the establishment of disease resistance, we generated a novel PR-1::Luciferase transgenic line which was deployed in an imaging based screen to uncover novel defence-related mutants. Approximately, 5000 ethylmethane sulfonate (EMS) lines and 30 000 activation tagged lines were generated and screened for enhanced LUC activity via ultra low light imaging.

Suggested Citation

  • A. Chini & S. Murray & J. Grant & C. Thomson & G. Loake, 2002. "Characterisation of a PR-1: Luciferase transgenic line deployed to uncover novel defence-related Arabidopsis mutants by luciferase imaging," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI2-6thCo), pages 615-616.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si2-6thconfefpp:id:10570-pps
    DOI: 10.17221/10570-PPS
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