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Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta

Author

Listed:
  • A.M. Hietala

    (Norwegian Forest Research Institute, 1432 Ås, Norway *)

  • M. Eikenes

    (Norwegian Forest Research Institute, 1432 Ås, Norway *)

  • M. Kvaalen

    (Norwegian Forest Research Institute, 1432 Ås, Norway *)

  • H. Solheim

    (Norwegian Forest Research Institute, 1432 Ås, Norway *)

  • C.G. Fossdal

    (Norwegian Forest Research Institute, 1432 Ås, Norway *)

Abstract

A quantitative multiplex real-time PCR procedure was developed to monitor the dynamics in Norway spruce-Heterobasidion annosum pathosystem. The assay reliably detected down to 1 pg of H. annosum DNA and 1 ng of host DNA in multiplex conditions. As a comparative method for quantifying fungal colonization, we applied the ergosterol assay. There was a very high correlation between the results obtained with the two methods, this strengthening the credibility of both assays. The advantages and disadvantages of these assays are discussed.

Suggested Citation

  • A.M. Hietala & M. Eikenes & M. Kvaalen & H. Solheim & C.G. Fossdal, 2002. "Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI2-6thCo), pages 406-407.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si2-6thconfefpp:id:10507-pps
    DOI: 10.17221/10507-PPS
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