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Selection of RNA isolation method for molecular detection of grapevine viruses

Author

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  • P. Komínek

    (Research Institute of Crop Production, 161 06 Prague-Ruzyně, Czech Republic)

Abstract

Grapevines infected with Grapevine leafroll-associated virus-1 (GLRaV-1) and Grapevine leafroll-associated virus-3 (GLRaV-3) were selected. Total RNA was isolated from grapevine phloem tissue scrapped from dormant canes by three different methods: extraction with urea buffer followed with phenol-chloroform extraction, method using ConcertTM reagent (Invitrogen) followed with chloroform-isopropylalcohol extraction, and procedure using RNeasy Plant Mini Kit (Qiagen). The highest yield of RNA was obtained using ConcertTM reagent. If this RNA was used in RT-PCR, GLRaV-1 and GLRaV-3 were easily detected. From RNA isolated by other two methods these viruses were not detected.

Suggested Citation

  • P. Komínek, 2002. "Selection of RNA isolation method for molecular detection of grapevine viruses," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI2-6thCo), pages 267-270.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si2-6thconfefpp:id:10463-pps
    DOI: 10.17221/10463-PPS
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