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Detection of Peach latent mosaic viroid by PCR

Author

Listed:
  • P. Ryšánek

    (65 21 Prague-Suchdol, Czech Republic *)

  • M. Zouhar

    (65 21 Prague-Suchdol, Czech Republic *)

  • M. Hassan

    (65 21 Prague-Suchdol, Czech Republic *)

Abstract

Peach latent mosaic viroid (PLMVd) is widespread in peach all over the world. It has never been reported from the Czech Republic. That is why we adapted specific and sensitive method for its detection, PCR, to be able to prove its possible occurrence and for certification purposes. Primers PLMVdR, PLMVdF1 and PLMVdF2 were designed on the basis of published RNA sequences. Products of amplification are 208 and 114 bp long for PLMVdF1 and PLMVdF2, respectively. Four PLMVd isolates from Dr Di Serio (CNR Bari) were used as standards. Potato spindle tuber viroid and Hop latent viroid infected plant material and also healthy material were used to check detection specifity. Both RNA extraction from plant material and PCR were optimalized so that this method of PLMVd detection can also be used for certification purposes.

Suggested Citation

  • P. Ryšánek & M. Zouhar & M. Hassan, 2002. "Detection of Peach latent mosaic viroid by PCR," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI2-6thCo), pages 249-251.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si2-6thconfefpp:id:10458-pps
    DOI: 10.17221/10458-PPS
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    Keywords

    PLMVd; PCR; peach; certification;
    All these keywords.

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