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Simplification of RNA preparation procedure for RT-PCR in detection of pome fruit tree viruses

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  • J.K. Kundu

    (Department of Virology, Research Institute of Crop Production, 161 06 Prague-Ruzyně, Czech Republic)

Abstract

A rapid, easy to handling and sensitive RNA preparation procedure, RNA release protocol was described here for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) by RT-PCR. Comparing total RNA extraction protocol, RNA release protocol give raised similar rate of ASPV and ASGV detection within the field-grown apple cultivars. Among sampling plant tissues, the bud leaf and leaf (during blossom) were showed efficient tissues for the routine detection, regardless the using RNA preparation procedures.

Suggested Citation

  • J.K. Kundu, 2002. "Simplification of RNA preparation procedure for RT-PCR in detection of pome fruit tree viruses," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI2-6thCo), pages 252-254.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si2-6thconfefpp:id:10457-pps
    DOI: 10.17221/10457-PPS
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    Keywords

    RT-PCR; ASPV; ASGV; RNA release;
    All these keywords.

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