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Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein

Author

Listed:
  • M. Paape

    (Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany)

  • S. Nell

    (Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany)

  • S. Von Bargen

    (Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany)

  • J.-W. Kellmann

    (Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany)

Abstract

To search for host proteins involved in systemic spreading of Tomato spotted wilt virus (TSWV), the virus-encoded NSm movement protein has been utilized as a bait in yeast two-hybrid interaction trap assays. J-domain chaperones from different host species and a protein denominated At-4/1 from Arabidopsis thaliana showing homologies to myosins and kinesins were identified as NSm-interacting partners. In this communication we illustrate that following TSWV infection, J-domain proteins accumulated in systemically infected leaves of A. thaliana, whereas At-4/1 was constitutively detected in leaves of A. thaliana and Nicotiana rustica.

Suggested Citation

  • M. Paape & S. Nell & S. Von Bargen & J.-W. Kellmann, 2002. "Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI1-6thCo), pages 108-111.
  • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si1-6thconfefpp:id:10331-pps
    DOI: 10.17221/10331-PPS
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