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Discrimination of Czech Armillaria species based on PCR method and high performance liquid chromatography

Author

Listed:
  • J. Lochman

    (Department of Biochemistry and)

  • O. Šerý

    (Department of Biochemistry and)

  • L. Jankovský

    (Department of Biochemistry and)

  • V. Mikes

    (Department of Biochemistry and)

Abstract

The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. We analysed about 40 isolates of Armillaria collected in Czech Republic by PCR and restriction analysis using gel electrophoresis and ion-exchange HPLC. Restrictase Hinf I was able to discriminate all investigated Armillaria species. The sensitivity and resolution of HPLC method was better than that performed by gel electrophoresis. HPLC was able to detect some heterozygous. The results prove the similarity of the species A. borealis, A. cepistipes, A. gallica, A. ostoyae in difference of A. mellea and A. tabescens.

Suggested Citation

  • J. Lochman & O. Šerý & L. Jankovský & V. Mikes, 2002. "Discrimination of Czech Armillaria species based on PCR method and high performance liquid chromatography," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI1-6thCo), pages 31-34.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si1-6thconfefpp:id:10316-pps
    DOI: 10.17221/10316-PPS
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