IDEAS home Printed from https://ideas.repec.org/a/caa/jnlpps/v38y2002isi1-6thconfefppid10314-pps.html
   My bibliography  Save this article

Molecular identification of a phytoplasma naturally infecting Populus nigra L. cv. Italica trees in Croatia

Author

Listed:
  • M. Šeruga

    (Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia)

  • D. Škorić

    (Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia)

  • S. Botti

    (Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia)

  • S. Paltrinieri

    (Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia)

  • N. Juretić

    (Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia)

  • A. Bertaccini

    (Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia)

Abstract

Leaf and branch samples of 10 Populus nigra L. cv. Italica trees were collected from the urban Zagreb area in late summer/autumn 2001. One of the trees exhibited leaf yellowing, overall sparse foliage, stunting and decline. Phytoplasma 16S rDNA was amplified in direct and nested PCR assays using universal and specific phytoplasma primer pairs, from nucleic acids extracted by two different procedures. Strong amplification signals were observed in samples from symptomatic Lombardy poplar as well as in samples from 4 of the asymptomatic trees. RFLP analyses of amplicons showed patterns characteristic of the phytoplasmas belonging to the Aster yellows group (16SrI). This is the first report of a phytoplasma naturally infecting poplar in Croatia.

Suggested Citation

  • M. Šeruga & D. Škorić & S. Botti & S. Paltrinieri & N. Juretić & A. Bertaccini, 2002. "Molecular identification of a phytoplasma naturally infecting Populus nigra L. cv. Italica trees in Croatia," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI1-6thCo), pages 28-30.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si1-6thconfefpp:id:10314-pps
    DOI: 10.17221/10314-PPS
    as

    Download full text from publisher

    File URL: http://pps.agriculturejournals.cz/doi/10.17221/10314-PPS.html
    Download Restriction: free of charge
    File URL: http://pps.agriculturejournals.cz/doi/10.17221/10314-PPS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/10314-PPS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlpps:v:38:y:2002:i:si1-6thconfefpp:id:10314-pps. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.