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Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV)

Author

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  • D. Vašková

    (*Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05 České Budějovice, Czech Republic)

  • J. Špak

    (*Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05 České Budějovice, Czech Republic)

Abstract

Control of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA by concurrent activity of a special enzyme mix (AMV-reverse transcriptase, RNaseH, T7 RNA polymerase) at a single temperature (41°C). Specific and sensitive detection of the amplified sequence can be performed in the same tube using molecular beacons. Sensitivity of SVBV-NASBA was 102 molecules of in vitro RNA detected per reaction. Results of the NASBA-based detection of SVBV in indicator strawberry plants were well comparable to the results of PCR.

Suggested Citation

  • D. Vašková & J. Špak, 2002. "Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV)," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI1-6thCo), pages 24-27.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si1-6thconfefpp:id:10313-pps
    DOI: 10.17221/10313-PPS
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