IDEAS home Printed from https://ideas.repec.org/a/caa/jnlpps/v35y1999i3id9703-pps.html
   My bibliography  Save this article

Serological and biochemical distinguishing of Pseudomonas syringae pathovars on peas

Author

Listed:
  • Iveta Pánková
  • Blanka Kokošková

Abstract

Polyclonal antisera to detect and determine two related pathovars, Pseudomonas syringae pv. pisi and Pseudomonas syringae pv. syringae, were prepared Untreated bacterial cells, or fixed by formaldehyde or glutaraldehyde were used as antigens. After cross-absorption with heterologous antigens, antisera revealed a high level of specificity in slide agglutination, Ouchterlony gel double-diffusion and in DAS-ELISA and PTA-ELISA. Each of 14 P s. pisi prepared polyclonal antisera could detect and deter­ mine all strains of P s. pisi, regardless of race. PTA-ELISA was the most appropriate serological test to distinguish Pseudomonas syringae pathovars on peas. Most of the P s. pisi strains from foreign collections were in serological tests confirmed asP. s. pisi, while most of the Czech strains suspected as P s. pisi were determined as P s. syringae strains. The principal biochemical reaction, i.e., use of DL-homoserine as a carbon source to grow P s. pisi but not P s. syringae , was proved not to be sufficiently reliable to distinguish both pathovars.

Suggested Citation

  • Iveta Pánková & Blanka Kokošková, 1999. "Serological and biochemical distinguishing of Pseudomonas syringae pathovars on peas," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 35(3), pages 79-84.
    • Handle: RePEc:caa:jnlpps:v:35:y:1999:i:3:id:9703-pps
    DOI: 10.17221/9703-PPS
    as

    Download full text from publisher

    File URL: http://pps.agriculturejournals.cz/doi/10.17221/9703-PPS.html
    Download Restriction: free of charge
    File URL: http://pps.agriculturejournals.cz/doi/10.17221/9703-PPS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/9703-PPS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlpps:v:35:y:1999:i:3:id:9703-pps. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.