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Assessment of eggplant germplasm genetic diversity using RAPD markers

Author

Listed:
  • Jelena Damnjanović

    (Research and Development Institute Tamiš, Pančevo, Serbia)

  • Zdenka Girek

    (Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia)

  • Svetlana Roljević Nikolić

    (Research and Development Institute Tamiš, Pančevo, Serbia)

  • Milan Ugrinović

    (Institute for Vegetable Crops Smederevska Palanka, Smederevska Palanka, Serbia)

  • Danica Mićanović

    (Serbian Chamber of Commerce and Industry, Bеlgrade, Serbia)

  • Suzana Pavlović

    (Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia)

Abstract

Evaluation of genetic resources of eggplant (Solanum melongena L.) from different geographical areas using molecular markers (RAPD) is of great importance in the breeding process. A total of 90 polymorphic amplified products were obtained from 10 decametric RAPD primers, used to analyse the genetic diversity of 20 genotypes of eggplant (16 local and 4 genotypes of foreign origin). The highest polymorphism was determined using the OPAF-16 primer (70.83%). The number of detected bands ranged from 13 (OPF-04) to 24 (OPAF-16), while the average number of bands per primer was 17.2. The lengths of the amplified fragments ranged from 400 to 9 000 bp. The value of the Jaccard's genetic distance coefficient ranged from 0.095 to 0.35, and the dendrogram constructed using the UPGMA method showed that 16 local and 4 foreign genotypes were grouped into nine groups (clusters). Populations K1, K8/1, K19, K22, K25 and K38 represent genotypes that separated from the others and formed single clusters. The lowest value of the calculated genetic distance was 0.095 between domestic genotypes K13 and K12, which also showed morphological similarity in terms of shape and colour. On the other hand, the highest value of genetic distance was calculated between foreign genotypes K19 and K25 (0.35), K19 and K34 (0.34) and K19 and K38 (0.34). Genetically distinct genotypes identified using RAPD markers could be potential starting genetic material for crossing with other genotypes to obtain new and improved eggplant varieties.

Suggested Citation

  • Jelena Damnjanović & Zdenka Girek & Svetlana Roljević Nikolić & Milan Ugrinović & Danica Mićanović & Suzana Pavlović, 2024. "Assessment of eggplant germplasm genetic diversity using RAPD markers," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 51(3), pages 212-218.
    • Handle: RePEc:caa:jnlhor:v:51:y:2024:i:3:id:90-2023-hortsci
    DOI: 10.17221/90/2023-HORTSCI
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    References listed on IDEAS

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    1. Suzana Pavlović & Jelena Damnjanović & Zdenka Girek & Lela Belić & Milan Ugrinović, 2024. "Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 60(1), pages 50-54.
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