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The effect of ellagic acid on rabbit sperm in vitro parameters after cryopreservation

Author

Listed:
  • Jovana Grba

    (University of Novi Sad, Faculty of Agriculture, Department of Animal Science, Serbia)

  • Lenka Kuželová

    (Faculty of Biotechnology and Food Science, Slovak University of Agriculture, Nitra, Slovak Republic
    AgroBioTech Research Centre, Slovak University of Agriculture, Nitra, Slovak Republic)

  • Alexander Makarevich

    (Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic)

  • Andrej Baláži

    (Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic)

  • Saša Dragin

    (University of Novi Sad, Faculty of Agriculture, Department of Animal Science, Serbia)

  • Dragana Tekić

    (University of Novi Sad, Faculty of Agriculture, Department of Agroeconomy, Serbia)

  • Peter Chrenek

    (Faculty of Biotechnology and Food Science, Slovak University of Agriculture, Nitra, Slovak Republic
    Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic)

Abstract

Cryopreservation reduces the antioxidant activity of spermatozoa and makes them more sensitive to damage caused by reactive oxygen species (ROS). The addition of antioxidants to the freezing medium could prevent cryo-damage by mitigating the harmful effects of ROS and, thus, protecting the spermatozoa. This study aimed to evaluate the effect of ellagic acid (EA) on the rabbit sperm traits after freezing-thawing. Semen samples collected from New Zealand White rabbit males were cryopreserved in a BotuCrio freezing medium (Nidacon, Sweden) supplemented with different concentrations of ellagic acid (EA at 0, 0.5, 1.5 and 2.5 mM) using the manual slow freezing procedure. After thawing, sperm motility parameters were evaluated by CASA. The parameters of viability (DRAQ7), apoptosis (Yo-Pro-1), acrosome integrity (peanut agglutinin; PNA), intracellular ROS (CellROX) and mitochondrial activity (MitoTracker) were evaluated by flow cytometry. EA added to the freezing medium at all concentrations led to a significant reduction (P < 0.05) in intracellular ROS in frozen-thawed sperm cells. However, this effect was not reflected in motility parameters. Semen supplemented with 1.5 mM EA also yielded a lower proportion of apoptotic cells compared to the control group. In conclusion, EA supplementation of semen extender demonstrated its antioxidative properties protecting spermatozoa against oxidative damage during cryopreservation. Nevertheless, to draw a definitive conclusion regarding the effect of EA on spermatozoa functionality, additional research is necessary.

Suggested Citation

  • Jovana Grba & Lenka Kuželová & Alexander Makarevich & Andrej Baláži & Saša Dragin & Dragana Tekić & Peter Chrenek, 2024. "The effect of ellagic acid on rabbit sperm in vitro parameters after cryopreservation," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 69(3), pages 110-117.
    • Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:3:id:142-2023-cjas
    DOI: 10.17221/142/2023-CJAS
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