IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v68y2023i1id93-2022-cjas.html
   My bibliography  Save this article

Label-free proteomics to identify keratins and keratin-associated proteins and their effects on the fleece traits of Inner Mongolia Cashmere Goats

Author

Listed:
  • Chongyan Zhang

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Yuchun Xie

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Juntao Guo

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Xin Su

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Cun Zhao

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Qing Qin

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Dongliang Dai

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Zhixin Wang

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Jinquan Li

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

  • Zhihong Liu

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding of Ministry of Agriculture, Hohhot, P.R. China)

Abstract

Inner Mongolia Cashmere Goat fleece is derived from a heterogeneous coat. The guard hair is produced by primary hair follicles, and the down hair is produced by secondary hair follicles. Reports have focused on research related to the different hair follicle types, but no related study has addressed the difference in the proteins of down hair (DH) and guard hair (GH), and whether the protein composition of these materials differs remains to be studied. The protein composition of DH and GH was studied. A total of 108 proteins were identified in DH, and 116 proteins were identified in GH; 39 proteins were differentially expressed, of which 18 proteins were upregulated and 21 proteins were downregulated in DH versus GH. In this study, through the measurement of DH and GH samples from Inner Mongolia Cashmere Goats, the diameter of DH relative to GH was downregulated (P < 0.05), a property that may be related to the KRT38 protein. Moreover, the strength of GH was significantly higher than that of DH (P < 0.05), a property that may be affected by the KRTAP8-1 protein. GO analysis showed that the different traits of DH and GH from Inner Mongolia Cashmere Goats are affected by keratin, actin, and calcium-binding proteins. This study uses a nonlabelled quantitative proteomics method to study the proteins in DH and GH, aiming to identify the keratin family in Inner Mongolia Cashmere Goats and to provide a new direction for studying DH and GH traits at the protein level.

Suggested Citation

  • Chongyan Zhang & Yuchun Xie & Juntao Guo & Xin Su & Cun Zhao & Qing Qin & Dongliang Dai & Zhixin Wang & Jinquan Li & Zhihong Liu, 2023. "Label-free proteomics to identify keratins and keratin-associated proteins and their effects on the fleece traits of Inner Mongolia Cashmere Goats," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 68(1), pages 17-29.
    • Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:1:id:93-2022-cjas
    DOI: 10.17221/93/2022-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/93/2022-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/93/2022-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/93/2022-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Keywords

    KRT1; KRT38; KRTAP8-1; strength; average diameter;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:68:y:2023:i:1:id:93-2022-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.