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Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells

Author

Listed:
  • Shaoyu Wang

    (College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China)

  • Kui Li

    (Zhejiang Animal Husbandry Techniques Extension Station, Hangzhou, P.R. China)

  • Hui Gao

    (Zhejiang Animal Husbandry Techniques Extension Station, Hangzhou, P.R. China)

  • Zepeng Liu

    (College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China)

  • Shuang Shi

    (College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China)

  • Qiang Tan

    (College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China)

  • Zhengguang Wang

    (College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China
    Huzhou Southern Taihu Lake Modern Agricultural Technology Center, Zhejiang University, P.R. China)

Abstract

Ubiquitin-specific protease 8 (USP8), a member of the ubiquitin-specific protease (USP) family, was originally identified as playing a role in the regulation of growth and cell cycle. However, its functional role in myogenesis is unknown. In this study, we investigated the role of USP8 in proliferation and differentiation of sheep skeletal muscle satellite cells. The results showed that the expression level of USP8 was significantly increased on days 2 and 3 following the induction of the differentiation process. Furthermore, knocking down USP8 resulted in a significant increase in myogenin-positive cells, and promoted early differentiation of satellite cells by regulating the expression level of paired box 7 (PAX7). Additionally, knocking down USP8 suppressed muscle satellite cell proliferation, possibly explaining that the relative mRNA level of USP8 was linearly related to muscle fibre density of Hu sheep. Overall, our research demonstrates that USP8 plays a role in proliferation and early differentiation of skeletal muscle satellite cells.

Suggested Citation

  • Shaoyu Wang & Kui Li & Hui Gao & Zepeng Liu & Shuang Shi & Qiang Tan & Zhengguang Wang, 2021. "Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 66(3), pages 87-96.
    • Handle: RePEc:caa:jnlcjs:v:66:y:2021:i:3:id:105-2020-cjas
    DOI: 10.17221/105/2020-CJAS
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    References listed on IDEAS

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    1. Haifeng Gu & Xingxing Shi & Chao Liu & Chaoqun Wang & Ning Sui & Yu Zhao & Jiaqi Gong & Fuping Wang & Hong Zhang & Wei Li & Tongbiao Zhao, 2019. "USP8 maintains embryonic stem cell stemness via deubiquitination of EPG5," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
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    More about this item

    Keywords

    myogenesis; myogenin; desmin; myosin heavy chain 3; PAX7;
    All these keywords.

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