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Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro

Author

Listed:
  • Francesco Vizzarri

    (Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy)

  • Marisa Palazzo

    (Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy)

  • Donato Casamassima

    (Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy)

  • Lubomir Ondruska

    (National Agricultural and Food Centre, Nitra, Slovak Republic)

  • Martin Massanyi

    (Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic)

  • Filip Tirpak

    (Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic)

  • Grzegorz Formicki

    (Institute of Biology, Pedagogical University, Krakow, Poland)

  • Agnieszka Gren

    (Institute of Biology, Pedagogical University, Krakow, Poland)

  • Peter Massanyi

    (Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic
    Institute of Biology, Pedagogical University, Krakow, Poland)

Abstract

Verbascoside, the main component of Lippia citriodora extract, is one of the most powerful free radical scavengers exhibiting a wide biological activity. In in vivo study 20 adult New Zealand white rabbit bucks were divided into two homogeneous groups, one control (CON) and one verbascoside-supplemented (0.1%) in feed mixture (EXP) and later in vitro effects of verbascoside on the motility aspects of rabbit spermatozoa were analysed. The spermatozoa concentration, ejaculate volume, spermatozoa motility, progressive motility, distance parameters, velocity parameters and type of spermatozoa movement were negatively affected by Lippia citriodora leaves extract after the first 4 weeks of dietary treatment, till the end of experiment (8 weeks). Four weeks after the suspension of feed additive supplementation, all spermatozoa traits values returned to the normality, and in line with CON group. For in vitro findings, ejaculates from 10 male New Zealand white bucks were collected using an artificial vagina. Then it was diluted in physiological saline solution containing different concentrations of verbascoside at the concentration of 0, 0.0024, 0.0219, 0.157, 120.0 mg/ml (Ctrl, VB1, VB2, VB3, VB4 groups, respectively), using a dilution ratio of 1 : 4. The obtained data proved that verbascoside at the concentration of 0.0024 and 0.0219 mg/ml had no adverse effect on spermatozoa. Additionally, we found that verbascoside at higher concentrations (0.157 and 120.0 mg/ml) significantly altered all the motility parameters analysed in the experiment. In conclusion a possible negative effect of verbascoside supplementation into feed mixture (0.1%) on semen quality parameters in rabbit bucks as well as in vitro can be stated, obviously considering that target organs of antioxidant activities of phenylpropanoid glycosides are various. In addition it has to be emphasized that the extract showed a reversible action, since the semen traits of treated animals returned to the normality after the dietary administration period.

Suggested Citation

  • Francesco Vizzarri & Marisa Palazzo & Donato Casamassima & Lubomir Ondruska & Martin Massanyi & Filip Tirpak & Grzegorz Formicki & Agnieszka Gren & Peter Massanyi, 2019. "Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 64(1), pages 1-10.
    • Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:1:id:35-2018-cjas
    DOI: 10.17221/35/2018-CJAS
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    References listed on IDEAS

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    1. G. Pastorelli & R. Rossi & C. Corino, 2012. "Influence of Lippia citriodora verbascoside on growth performance, antioxidant status, and serum immunoglobulins content in piglets," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 57(7), pages 312-322.
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