IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v63y2018i8id24-2018-cjas.html
   My bibliography  Save this article

Effects of selenium on selenoprotein synthesis and antioxidant parameters of bovine mammary epithelial cells

Author

Listed:
  • Yongmei Guo

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China)

  • Jian Gong

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    College of Life Science and Technology, Inner Mongolia Normal University, Hohhot, P.R. China)

  • Binlin Shi

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China)

  • Xiaoyu Guo

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China)

  • Sumei Yan

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China)

Abstract

This study aimed to investigate the effects of selenium (Se) on the selenoproteins synthesis and antioxidant parameters of bovine mammary epithelial cells (BMECs). The experiment was conducted as a single factor completely randomized design to explore the effect of different levels of Se supplementation (0, 10, 20, 50, and 100 nmol/l) on selenoproteins synthesis and antioxidant parameters of BMECs, and to screen the appropriate dose of Se supplementation ensuring a better antioxidant function. Se supplementation increased cell proliferation, the activities of glutathione peroxidase (GPx) and superoxide dismutase, total antioxidant capacity and seleoprotein P (SelP) content, and decreased reactive oxygen species and malondialdehyde levels in a dose-dependent manner. Se supplementation of 50-100 nmol/l had a better effect. Se supplementation also increased thioredoxin reductase (TrxR) activity in a dose-dependent manner, and Se supplementation of 20-50 nmol/l had a better promoting effect. The dose-dependent response between Se supplementation and mRNA and protein expression of GPx1 and TrxR1, as well as SelP mRNA expression was also observed in this experiment. The mRNA and protein expression of GPx1 was up-regulated with the addition of 50-100 nmol/l Se, and the mRNA expression of TrxR1 and SelP was up-regulated with the addition of 20-100 nmol/l Se. Results indicated that Se supplementation of 50 nmol/l had a better promoting effect on the selenoproteins synthesis and antioxidant parameters of BMECs.

Suggested Citation

  • Yongmei Guo & Jian Gong & Binlin Shi & Xiaoyu Guo & Sumei Yan, 2018. "Effects of selenium on selenoprotein synthesis and antioxidant parameters of bovine mammary epithelial cells," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(8), pages 313-322.
    • Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:8:id:24-2018-cjas
    DOI: 10.17221/24/2018-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/24/2018-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/24/2018-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/24/2018-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Ana Stupin & Anita Cosic & Sanja Novak & Monika Vesel & Ivana Jukic & Brigita Popovic & Krunoslav Karalic & Zdenko Loncaric & Ines Drenjancevic, 2017. "Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas," IJERPH, MDPI, vol. 14(6), pages 1-15, June.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:63:y:2018:i:8:id:24-2018-cjas. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.