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Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm

Author

Listed:
  • M. Ziaullah

    (Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • A. Ijaz

    (Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • M. Aleem

    (Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • A.K. Mahmood

    (Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • H. Rehman

    (Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • S. Bhatti

    (Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan)

  • U. Farooq

    (University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan)

  • M.U. Sohail

    (Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan)

Abstract

The study was conducted to evaluate the potential cryoprotective effect of butylated hydroxytoluene (BHT) through post-thaw evaluation of canine semen and its optimal inclusion level. Ejaculated canine semen was extended in TRIS-glucose egg yolk extender containing various concentrations of BHT (0.5, 1.0, 1.5, 2.0, and 2.5mM). Semen was frozen at -196°C using 200 × 106 spermatozoa per 0.5 ml straws and post-thaw evaluation was carried out in terms of sperm motility, viability, plasma membrane integrity, and acrosomal integrity through phase-contrast microscope, supravital staining, hypo-osmotic swelling test, and normal acrosomal ridge, respectively. BHT was found to improve (P > 0.005) all post-thawed semen quality parameters at an inclusion level of 1.0mM in the extended semen. However, higher concentrations than this were found to have detrimental effects.

Suggested Citation

  • M. Ziaullah & A. Ijaz & M. Aleem & A.K. Mahmood & H. Rehman & S. Bhatti & U. Farooq & M.U. Sohail, 2012. "Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 57(8), pages 377-381.
    • Handle: RePEc:caa:jnlcjs:v:57:y:2012:i:8:id:6273-cjas
    DOI: 10.17221/6273-CJAS
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