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Effects of inhibiting nitric oxide synthase on cumulus expansion and nuclear maturation of sheep oocytes

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  • M. Heidari Amale

    (Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)

  • A. Zare Shahne

    (Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)

  • A. Abavisani

    (Physiology Section, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
    Embryonic and Stem Cell Biology and Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran)

  • S. Nasrollahi

    (Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)

Abstract

Nitric oxide (NO) is a biological signaling molecule that plays a crucial role in oocyte maturation of mammalians. It is generated by the nitric oxide synthase (NOS) enzyme from l-arginine. Although the effect of NO has been shown in oocyte maturation of some species, there is no report about its effect on the in vitro maturation of sheep oocyte. So, this study aimed to investigate the importance of NO/NOS system in the in vitro maturation of ovine oocytes. Different concentrations of L-NAME (a NOS inhibitor) (0.1, 1 and 10mM) were added to maturation medium to evaluate the effect of inhibiting NOS on cumulus expansion and meiotic resumption of sheep oocytes. After 26 h culture, low and medium concentrations of L-NAME (0.1 and 1mM) had no significant effect on cumulus expansion, however, its higher concentration (10mM) decreased percentage of oocytes with total cumulus expansion as compared to control (P < 0.05). The extrusion of the first polar body was also suppressed in a dose-dependent manner, so that the addition of 10mM L-NAME to maturation medium significantly stopped oocytes in GV stage (P < 0.05). Moreover, to confirm the results and to evaluate if this effect is reversible, 0.1mM sodium nitroprusside (SNP, a NO donor) was added only to the maturation medium which had the highest concentration of L-NAME (10mM). The concomitant addition of NOS inhibitor with NO donor reversed the inhibitory effect of L-NAME on cumulus expansion and meiotic maturation. These results indicated that NO/NOS system is involved in the maturation of sheep oocytes.

Suggested Citation

  • M. Heidari Amale & A. Zare Shahne & A. Abavisani & S. Nasrollahi, 2011. "Effects of inhibiting nitric oxide synthase on cumulus expansion and nuclear maturation of sheep oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(6), pages 284-291.
    • Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:6:id:1284-cjas
    DOI: 10.17221/1284-CJAS
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    1. E. Chmelíková & M. Sedmíková & J. Petr & T. Kott & V. Lánská & L. Tůmová & H. Tichovská & M. Ješeta, 2009. "Expression and localization of nitric oxide synthase isoforms during porcine oocyte growth and acquisition of meiotic competence," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(4), pages 137-149.
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