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Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA

Author

Listed:
  • L. Vostrý

    (Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science Prague, Prague, Czech Republic
    Institute of Animal Science Praha Uhříněves, Prague, Czech Republic)

  • O. Kracíková

    (Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science Prague, Prague, Czech Republic)

  • B. Hofmanová

    (Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science Prague, Prague, Czech Republic)

  • V. Czerneková

    (Institute of Animal Science Praha Uhříněves, Prague, Czech Republic)

  • T. Kott

    (Institute of Animal Science Praha Uhříněves, Prague, Czech Republic)

  • J. Přibyl

    (Institute of Animal Science Praha Uhříněves, Prague, Czech Republic
    Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science Prague, Prague, Czech Republic)

Abstract

The Old Kladruber horse is the oldest original Czech horse breed - gene resource, with pedigree records spanning three centuries. Because the population is closed, there is a concern about the loss of genetic variation. The genetic diversity within and amongst sire lines was evaluated using microsatellite markers and based on pedigree information. The DNA analysis, covering 16 microsatellite loci, revealed that the genetic diversity (observed heterozygosity = 0.637, genetic diversity = 0.678 and the mean number of alleles = 8.25) for the Old Kladruber horse is consistent with other populations of horses. Inter-line genetic difference in sire lines reached the mean value (the FST values ranged from 0.020 to 0.017). Differences amongst the sire lines were identified using genetic distances and principal component analysis. One gene cluster comprised black variation sire lines, while the second cluster included the sire lines of the grey variation. In the subjects monitored, the average inbreeding coefficient of 0.076 was estimated on the basis of pedigree information. The highest mean values of the coefficient of relationship were estimated within the colour variations. When comparing the genetic distance and the average coefficient of relationship, differences were found out. These results are useful for the development of breeding strategies which consider classical horse breeding as well as recent achievements of population and conservation genetics.

Suggested Citation

  • L. Vostrý & O. Kracíková & B. Hofmanová & V. Czerneková & T. Kott & J. Přibyl, 2011. "Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(4), pages 163-175.
  • Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:4:id:1437-cjas
    DOI: 10.17221/1437-CJAS
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    References listed on IDEAS

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    1. S. Kusza & E. Gyarmathy & J. Dubravska & I. Nagy & A. Jávor & S. Kukovics, 2009. "Study of genetic differences among Slovak Tsigai populations using microsatellite markers," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(10), pages 468-474.
    2. R.J. Chen & Z.P. Yang & D.J. Ji & D.Y. Qu & Y.L. Li & Y.J. Mao & D.L. Huang, 2009. "Analysis of genetic polymorphism in six meat sheep breeds and genetic distances between them," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(10), pages 461-467.
    3. J. Zrůstová & A. Knoll & T. Urban & S. Čepica, 2009. "The visfatin (NAMPT; PBEF1) gene polymorphisms and associations with meat performance traits in three pig breeds kept in the Czech Republic," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(10), pages 443-447.
    4. W.B. Bao & J.T. Shu & X.S. Wu & H.H. Musa & C.L. Ji & G.H. Chen, 2009. "Genetic diversity and relationship between genetic distance and geographical distance in 14 Chinese indigenous chicken breeds and red jungle fowl," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(2), pages 74-83.
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    Cited by:

    1. B. Hofmanová & L. Vostrý & I. Majzlík & H. Vostrá-Vydrová, 2015. "Characterization of greying, melanoma, and vitiligo quantitative inheritance in Old Kladruber horses," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 60(10), pages 443-451.
    2. L. Vostrý & B. Hofmanová & H. Vostrá Vydrová & J. Přibyl & I. Majzlík, 2012. "Estimation of genetic parameters for melanoma in the Old Kladruber horse," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 57(2), pages 75-82.
    3. Hana Vostrá-Vydrová & Luboš Vostrý & Barbora Hofmanová & Nina Moravčíková & Zdeňka Veselá & Irena Vrtková & Alexandra Novotná & Radovan Kasarda, 2018. "Genetic diversity and admixture in three native draught horse breeds assessed using microsatellite markers," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(3), pages 85-93.
    4. Barbora Hofmanová & Luboš Vostrý & Hana Vostrá-Vydrová & Adéla Dokoupilová & Ivan Majzlík, 2019. "Estimation of genetic and non-genetic effects influencing coat colour in black horses," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 64(1), pages 41-48.
    5. L. Vostrý & Z. Čapková & J. Přibyl & K. Mach, 2011. "Analysis of Czech cold-blooded horses: genetic parameters, breeding value and the influence of inbreeding depression on linear description of conformation and type characters," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(5), pages 217-230.

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