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Effects of a probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats

Author

Listed:
  • L. Strojný

    (Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic)

  • A. Bomba

    (Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic)

  • E. Hijová

    (Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic)

  • A. Chmelárová

    (Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic)

  • G. Mojžišová

    (Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic)

  • I. Bertková

    (Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic)

  • J. Koprovičová

    (Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic)

  • M. Pomfy

    (Institute of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic)

  • V. Strompfová

    (Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic)

  • M. Molokáčová

    (Department of Microbiology, Teaching Hospital, Košice, Slovak Republic)

Abstract

Effects of the probiotic (PRO) Lactobacillus plantarum and of the combination of PRO and the prebiotic (PRE) inulin enriched with oligofructose (2%), and PRO with Lini oleum virginale (O) on counts of lactobacilli and coliforms and enzymatic activities in faeces of rats were studied. The rats (n = 60) were divided into 5 groups of 12 subjects. The animals were fed on a high fat diet (10%) for 8 weeks of experiment. Colon cancer was induced by the application of 1,2-dimethylhydrazine (DMH) twice a week in a dose of 20 mg/kg s.c. in groups G2-G5. The rats in group 1 (control 1) received a diet without any supplements. The rats in group 2 (control 2) received 1,2 DMH without any supplements. The rats in group 3 received PRO, group 4 PRO and PRE, and group 5 received PRO and O. A significant decrease (P < 0.05) of coliforms was found out after the application of PRO, PRO-O, and PRO-PRE in comparison with control group G2. Significantly higher (P < 0.05) counts of lactobacilli were determined after the application of PRO-O and PRO-PRE. Significantly lower (P < 0.001) activities of β-galactosidase, β-glucuronidase and α-glucosidase were observed in PRO, PRO-PRE and PRO-O, while in the case of the enzyme β-glucosidase the activity was lower only after the addition of PRO-O. The protective effect of lactobacilli was observed in the order PRO-O, PRO-PRE, and PRO. It was shown that combinations of PRO-O and PRO-PRE had a synergistic effect which was higher than the effect of administering only PRO.

Suggested Citation

  • L. Strojný & A. Bomba & E. Hijová & A. Chmelárová & G. Mojžišová & I. Bertková & J. Koprovičová & M. Pomfy & V. Strompfová & M. Molokáčová, 2011. "Effects of a probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(3), pages 99-106.
    • Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:3:id:56-2010-cjas
    DOI: 10.17221/56/2010-CJAS
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    References listed on IDEAS

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    1. R. Herich & T. Kokinčáková & A. Lauková & M. Levkutová, 2010. "Effect of preventive application of Enterococcus faecium EF55 on intestinal mucosa during salmonellosis in chickens," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(1), pages 42-47.
    2. M. Marciňáková & A. Lauková & M. Simonová & V. Strompfová & B. Koréneková & P. Naď, 2008. "A new probiotic and bacteriocin-producing strain of Enterococcus faecium EF9296 and its use in grass ensiling," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 53(8), pages 335-344.
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