IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v54y2009i7id1722-cjas.html
   My bibliography  Save this article

Differentiation of porcine wild-type lactobacilli strains, with ERIC-PCR and PFGE band patterns included in polyphasic taxonomy

Author

Listed:
  • E. Bolado-Martínez

    (Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a la Victoria, Hermosillo, Sonora, México)

  • E. Acedo-Félix

    (Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a la Victoria, Hermosillo, Sonora, México)

Abstract

Probiotic administration is an alternative to antibiotic supplementation in pig management. However, potential probiotic microorganisms included in foods must be well defined and correctly named, on the basis of a valid taxonomic system. The aim of the present study was to analyze band patterns generated by the polymerase chain reaction (PCR) amplification of enterobacterial repetitive intergenic consensus sequences (ERIC-PCR), obtained from porcine wild-type Lactobacillus strains, as a rapid alternative for genotypic characterization in polyphasic taxonomy. In the present study 36 porcine wild-type Lactobacillus strains were analyzed by polyphasic taxonomy which included API 50 CHL system, ERIC-PCR, and pulsed field gel electrophoresis (PFGE) band analysis, after digestion with XbaI or SpeI restriction enzymes. Polyphasic taxonomy discriminated among 23 strains of Lactobacillus reuteri, 12 strains of Lactobacillus salivarius and one strain of Lactobacillus mucosae. None of the tested methods was able to reliably resolve the three selected species of lactobacilli at a strain level. However, results improved considerably when ERIC-PCR results were combined with phenotypic characterization, and those results were comparable to the taxonomy that included PFGE.

Suggested Citation

  • E. Bolado-Martínez & E. Acedo-Félix, 2009. "Differentiation of porcine wild-type lactobacilli strains, with ERIC-PCR and PFGE band patterns included in polyphasic taxonomy," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(7), pages 307-314.
    • Handle: RePEc:caa:jnlcjs:v:54:y:2009:i:7:id:1722-cjas
    DOI: 10.17221/1722-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/1722-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/1722-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/1722-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:54:y:2009:i:7:id:1722-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.