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Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens

Author

Listed:
  • M. Simon

    (Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic)

  • J. Antalíková

    (Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic)

  • Ľ. Horovská

    (Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic)

  • J. Jankovičová

    (Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic)

  • K. Fábryová

    (Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic)

  • S. Hluchý

    (Slovak University of Agriculture, Nitra, Slovak Republic)

  • P. Chrenek

    (Slovak University of Agriculture, Nitra, Slovak Republic
    Slovak Agricultural Research Centre, Research Institute of Animal Production, Nitra, Slovak Republic)

  • V. Tančin

    (Slovak Agricultural Research Centre, Research Institute of Animal Production, Nitra, Slovak Republic)

Abstract

Studies that involved testing monoclonal antibodies (mAbs) for cross-species reactivity proved to be efficient for the identification of previously unrecognized antigens in a number of different species. Twenty-six mAbs specific to different bovine CD (cluster defined) antigens (CD9, CD18, CD45R, CD41/61, CD62L, MHC class I and bovine IgG light chain molecule) were assayed for reactivity with rabbit peripheral blood leukocytes. Four of the mAbs recognizing CD9 and CD41/61 were reactive with rabbit platelets or granulocytes. These were investigated further by immunoblotting and immunohistochemical staining. The study identified CD9 and CD41/61 molecules on rabbit cells by mAbs IVA-50 and IVA-38. It showed that IVA-50 is a new valuable CD9 reagent for rabbit immunology which could be used for immunofluorescence staining or ELISA assay, immunohistological and molecular studies of rabbit CD9 antigen. IVA-38 recognizes the CD41/61 on rabbit platelets in indirect immunofluorescence and ELISA assay.

Suggested Citation

  • M. Simon & J. Antalíková & Ľ. Horovská & J. Jankovičová & K. Fábryová & S. Hluchý & P. Chrenek & V. Tančin, 2009. "Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(6), pages 270-276.
    • Handle: RePEc:caa:jnlcjs:v:54:y:2009:i:6:id:1728-cjas
    DOI: 10.17221/1728-CJAS
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    References listed on IDEAS

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    1. M. Simon & S. Hluchý & Ľ. Horovská & J. Antalíková & J. Čuboň, 2007. "Immunohistochemical localization of adhesion molecules (CD62 and CD18) in the mammary gland of dairy cows," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(4), pages 88-95.
    2. J. Valentovičová & M. Simon & J. Antalíková, 2005. "Function of complement regulatory proteins in immunity of reproduction: a review," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(4), pages 135-141.
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      Keywords

      blood cells; tissue; CD9; CD41/61;
      All these keywords.

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