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Evaluation of parentage testing in the Czech population of Holsteincattle

Author

Listed:
  • V. Řehout

    (Agricultural Faculty, University of South Bohemia, České Budějovice, Czech Republic)

  • E. Hradecká

    (Agricultural Faculty, University of South Bohemia, České Budějovice, Czech Republic)

  • J. Čítek

    (Agricultural Faculty, University of South Bohemia, České Budějovice, Czech Republic)

Abstract

A set of 233 Holstein calves, their 233 dams and 44 sires from 4 large-sized herds was genotyped for 10 microsatellites recommended by ISAG for paternity/parentage testing. Heterozygosity of microsatellites ranged from 0.607 (SPS115) to 0.835 (TGLA227), and PIC from 0.575 (SPS115) to 0.816 (TGLA227) confirming a high polymorphism of each analysed locus. Their combined exclusion probability reached 0.999, which made them suitable and sufficiently accurate for parentage testing. A conflict between putative parents and calf in at least 2 markers with combined exclusion probability > 0.9 was required to reject parentage. The pedigree was considered incorrect in 25 (10.73%) out of the evaluated progeny/parent trios, of which in 10 samples the genotype of both parents did not match their offspring, and in 2 samples the putative dam was in conflict with the calf genotype. This result shows that the interchange of calves on farms with large-sized herds plays the role as important as the errors in sire identification, or recording mistakes.

Suggested Citation

  • V. Řehout & E. Hradecká & J. Čítek, 2006. "Evaluation of parentage testing in the Czech population of Holsteincattle," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(12), pages 503-509.
    • Handle: RePEc:caa:jnlcjs:v:51:y:2006:i:12:id:3970-cjas
    DOI: 10.17221/3970-CJAS
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    References listed on IDEAS

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    1. V. Czerneková & T. Kott & G. Dudková & Z. Sztankóová & J. Soldát, 2006. "Genetic diversity between seven Central European cattle breeds as revealed by microsatellite analysis," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(1), pages 1-7.
    2. J. Přibyl & P. Šafus & M. Štípková & L. Stádník & V. Čermák, 2004. "Selection index for bulls of Holstein cattle in the Czech Republic," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 49(6), pages 244-256.
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    Cited by:

    1. J. Stevanovic & Z. Stanimirovic & V. Dimitrijevic & M. Maletic, 2010. "Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(6), pages 221-226.

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