IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v50y2005i9id4226-cjas.html
   My bibliography  Save this article

Cathepsin involvement in muscle proteolysis in meat-type bulls

Author

Listed:
  • S.J. Rosochacki

    (Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland
    Department of Sanitary Biology and Biotechnology, Faculty of Building and Environmental Engineering, Białystok Technical University, Białystok, Poland)

  • T. Sakowski

    (Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland)

  • E. Juszczuk-Kubiak

    (Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland)

  • A. Butarewicz

    (Department of Sanitary Biology and Biotechnology, Faculty of Building and Environmental Engineering, Białystok Technical University, Białystok, Poland)

  • J. Połoszynowicz

    (Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland)

Abstract

Measurements were done of some lysosomal proteolytic enzyme activities involved in skeletal muscle proteolysis of the masculus longissimus lumborum et thoracis muscle (MLLT) of bulls. Samples from the same region between the 11th and 13th vertebra were taken after slaughter from Limousin (n = 10), Hereford (n = 10), Charolais (n = 10), Angus (n = 11) and Simmental (n = 11) bulls about 15 months old fed complete diet ad libitum. The activity of cathepsin D was determined as pepstatin (cathepsin D inhibitor) sensitive activity (PSCatD) towards 1% haemoglobin. Pepstatin-insensitive acid (PIA) and leupeptin-insensitive (thiol proteinases inhibitor) acid (LIA) autolytic activities were measured in the presence of 1mM Mg++. MLLT was also analysed for RNA, DNA and protein variables. The data were processed by analysis of variance. The highest activities in PSCatD (P ≤ 0.05), AAA (P ≤ 0.01) and LIA (P ≤ 0.05) as well as percentage of inhibition by pepstatin in cathepsin D (P ≤ 0.01) were estimated in Angus bulls, and the lowest in Limousin ones. These breeds differed in the above-mentioned activities by 20.3, 21.1, 31.1 and 13.1%, respectively. RNA/g of tissue was highest in Hereford and lowest in Limousin bulls (by about 15.3%, P ≤ 0.01). Similar differences (14.3%) were between Charolais and Limousin (P ≤ 0.01). CPS (103 RNA/protein) was higher by 18.3% (P ≤ 0.01) in Charolais compared to the value in Simmental bulls; similar differences were between Hereford and Simmental (16.4%, P ≤ 0.01). The DNA concentration was highest in Hereford (by about 30%) compared to Charolais bulls. Protein/103DNA ratio (mg/mg) - FCS - was higher by 33.4% in Charolais compared toHereford; RNA/DNA ratio was higher by 40.2% in Charolais compared toLimousin bulls. These results indicate the fast turnover of proteins in the groups of examined bulls and it can be concluded that in hypertrophic MLLT of bulls an anabolic decrease in degradation occurred.

Suggested Citation

  • S.J. Rosochacki & T. Sakowski & E. Juszczuk-Kubiak & A. Butarewicz & J. Połoszynowicz, 2005. "Cathepsin involvement in muscle proteolysis in meat-type bulls," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(9), pages 422-429.
    • Handle: RePEc:caa:jnlcjs:v:50:y:2005:i:9:id:4226-cjas
    DOI: 10.17221/4226-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/4226-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/4226-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/4226-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. S. J Rosochacki & T. Sakowski & J. Połoszynowicz & E. Juszczuk-Kubiak & A. Kowalik-Krupa & J. Oprządek, 2004. "Lysosomal proteolysis in skeletal muscles of bulls," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 49(8), pages 340-348.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:50:y:2005:i:9:id:4226-cjas. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.