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Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation

Author

Listed:
  • A. Kamalak

    (Kahramanmaras Sutcu Imam University, Faculty of Agriculture, Department of Animal Science, Kahrmanmaras, Turkey)

  • O. Canbolat

    (Bursa Uludag University, Faculty of Agriculture, Department of Animal Science, Bursa, Turkey)

  • Y. Gurbuz

    (Kahramanmaras Sutcu Imam University, Faculty of Agriculture, Department of Animal Science, Kahrmanmaras, Turkey)

  • O. Ozay

    (Kahramanmaras Sutcu Imam University, Faculty of Agriculture, Department of Animal Science, Kahrmanmaras, Turkey)

Abstract

Dry matter (DM) degradation of wheat straw (WS), barley straw (BS), lucerne hay (LH) and maize silage (MS) was determined using two different techniques: (i) in vitro gas production and (ii) nylon bag degradability technique. In vitro gas production and in situ DM disappearance were measured after 3, 6, 12, 24, 48, 72 and 96 hours of incubation. In situ and in vitro DM degradation kinetics was described using the equation y = a + b (1 - ect). In all incubations there were significant (P < 0.001) correlations between gas production and in situ DM disappearance or estimated parameters ((a + b)gas and (a + b)is or (a + b)gas and EDMDis) whereas there were no significant (P > 0.05) correlations between cgas and cis or bgas and bis. Gas production from the insoluble fraction (b) alone explained 98.3% of the variation of EDMD. The inclusion of gas production from the quickly soluble fraction (a) and rate constant (c) of gas production in the regression equation improved the accuracy of EDMD prediction. The correlations between the results of both methodologies seem to be sufficiently strong to predict degradability parameters from gas production parameters. It was concluded that the in vitro gas production technique has good potentiality to predict in situ DM disappearance and some DM degradation parameters.

Suggested Citation

  • A. Kamalak & O. Canbolat & Y. Gurbuz & O. Ozay, 2005. "Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(2), pages 60-67.
    • Handle: RePEc:caa:jnlcjs:v:50:y:2005:i:2:id:3996-cjas
    DOI: 10.17221/3996-CJAS
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    Citations

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    Cited by:

    1. L. Kolář & M. Maršálek & J. Frelich & S. Kužel & P. Smetana & J. Zedníková & M. Švecová, 2009. "Changes in methane release from organic matter passing through the digestive tract of horses," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(3), pages 112-120.
    2. F. Jančík & V. Koukolová & P. Kubelková & B. Čermák, 2009. "Effects of grass species on ruminal degradability of silages and prediction of dry matter effective degradability," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(7), pages 315-323.
    3. F. Jančík & V. Koukolová & P. Homolka, 2010. "Ruminal degradability of dry matter and neutral detergent fibre of grasses," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(9), pages 359-371.
    4. L. Kolář & J. Frelich & J. Brouček & S. Kužel & J. Borová-Batt & J. Peterka & M. Šlachta & K. Volfová & J. Pezlarová & J. Hřebečková & V. Čechová, 2010. "Anaerobic degradability of organic matter of cattle faeces and a possibility of its utilization," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(12), pages 538-547.

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