IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v55y2019i3id61-2018-cjgpb.html
   My bibliography  Save this article

Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids

Author

Listed:
  • Evangelia Stavridou

    (Department of Genetics and Plant Breeding, School of Agriculture, Forestry and Natural Environment, Thessaloniki, Greece
    Institute of Applied Biosciences, Thessaloniki, Greece)

  • Nikoleta A. Τzioutziou

    (Department of Genetics and Plant Breeding, School of Agriculture, Forestry and Natural Environment, Thessaloniki, Greece
    Division of Plant Sciences, College of Life Sciences, University of Dundee, Invergowrie, Dundee, UK)

  • Panagiotis Madesis

    (Institute of Applied Biosciences, Thessaloniki, Greece)

  • Nikolaos E. Labrou

    (Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece)

  • Irini Nianiou-Obeidat

    (Department of Genetics and Plant Breeding, School of Agriculture, Forestry and Natural Environment, Thessaloniki, Greece)

Abstract

The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast, Don Jose (92.5%) and Siena (83.3%) performed better with 0.5 mg/l Z and 0.1 mg/l IAA. The three hybrids did not differ in micropropagation index, however, Felina showed the highest number of in vitro rooted and in vivo acclimatized plants. Factors such as the age of explant, days in pre- and co-culture and the concentrations of acetosyringone and thiamine on Agrobacterium-mediated genetic transformation were assessed. The transformation indices were 37.04% for the Felina, 13.8% for Siena and 8.33% for Don Jose. We conclude that targeted genotype-specific regeneration protocols will provide an efficient and cost effective genetic transformation system for rootstock production and further incorporation into micropropagation and transgrafting systems.

Suggested Citation

  • Evangelia Stavridou & Nikoleta A. Τzioutziou & Panagiotis Madesis & Nikolaos E. Labrou & Irini Nianiou-Obeidat, 2019. "Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 55(3), pages 120-127.
    • Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:3:id:61-2018-cjgpb
    DOI: 10.17221/61/2018-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/61/2018-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/61/2018-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/61/2018-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:55:y:2019:i:3:id:61-2018-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.