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Development of a muskmelon cultivar with improved resistance to gummy stem blight and desired agronomic traits using gene pyramiding

Author

Listed:
  • Ning ZHANG

    (State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China)

  • Bing-Hua XU

    (Huaiyin Institute of Agricultural Sciences of Xuhuai Region, Jiangsu P.R. China)

  • Yan-Fei BI

    (State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China)

  • Qun-Feng LOU

    (State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China)

  • Jin-Feng CHEN

    (State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China)

  • Chun-Tao QIAN

    (State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China)

  • Yong-Bing ZHANG

    (Center of Hami Melon, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, P.R. China$2)

  • Hong-Ping YI

    (Center of Hami Melon, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, P.R. China$2)

Abstract

Gummy stem blight (GSB), caused by Didymella bryoniae (Auersw.) Rehm., is a severe disease affecting Cucurbitaceae crops including melons. The resistance of current melon varieties that carry a single Gsb resistance gene is insufficient to protect against the abundant variation of the D. bryoniae isolates. Pyramiding multiple Gsb resistance genes into melon cultivars is an effective way to develop a broad resistance spectrum and to increase the duration of GSB resistance. In this study, two resistance genes (Gsb-4 and Gsb-6) from two resistant accessions, PI482398 and PI420145, were pyramided into one variety using marker-assisted selection (MAS). The donor parent 4598 that contained Gsb-4 and Gsb-6 was hybridized with muskmelon Baipicui to produce BC1F1 and BC2F1. Phenotyping and MAS enabled identification and pyramiding of two Gsb genes in individuals of F1, BC1F1 and BC2F1. Field cultivation showed that individuals carrying both Gsb-4 and Gsb-6 had improved resistance to GSB and improved fruit quality. The results indicated that MAS-based pyramiding is an effective strategy for breeding melon cultivars with increased resistance to GSB.

Suggested Citation

  • Ning ZHANG & Bing-Hua XU & Yan-Fei BI & Qun-Feng LOU & Jin-Feng CHEN & Chun-Tao QIAN & Yong-Bing ZHANG & Hong-Ping YI, 2017. "Development of a muskmelon cultivar with improved resistance to gummy stem blight and desired agronomic traits using gene pyramiding," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 53(1), pages 23-29.
    • Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:84-2016-cjgpb
    DOI: 10.17221/84/2016-CJGPB
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