IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v52y2016i3id134-2015-cjgpb.html
   My bibliography  Save this article

Callus induction and frond regeneration in Spirodela polyrhiza

Author

Listed:
  • Youru WANG

    (Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, P.R. China
    Life Science College, Hubei Normal University, Huangshi, Hubei, P.R. China)

Abstract

Spirodela polyrhiza belongs to the family Lemnaceae (duckweed), which is a group of small aquatic plants offering an attractive plant expression system for the production of recombinant protein. No frond regeneration protocol has been established in this species yet. An efficient protocol for plant regeneration through organogenesis has been developed in Spirodela polyrhiza for the first time. Calli were successfully induced from 92% of explants on Murashige and Skoog (MS) medium with 10 μM naphthaleneacetic acid, 2 μM thidiazuron, 1μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 3% sucrose. MS medium containing 1% (m/v) sorbitol and 1 µM 2,4-D supported long lasting growth (at least 5 months) of 98% of calli. Plants regenerated from 92% of calli on Schenk and Hildebrandt (SH) medium with 10 μM zeatin and 1% (m/v) sucrose. The protocol for frond regeneration could be a good basis for transgenic engineering of S. polyrhiza.

Suggested Citation

  • Youru WANG, 2016. "Callus induction and frond regeneration in Spirodela polyrhiza," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 52(3), pages 114-119.
    • Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:3:id:134-2015-cjgpb
    DOI: 10.17221/134/2015-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/134/2015-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/134/2015-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/134/2015-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. W. Wang & G. Haberer & H. Gundlach & C. Gläßer & T. Nussbaumer & M.C. Luo & A. Lomsadze & M. Borodovsky & R.A. Kerstetter & J. Shanklin & D.W. Byrant & T.C. Mockler & K.J. Appenroth & J. Grimwood & J., 2014. "The Spirodela polyrhiza genome reveals insights into its neotenous reduction fast growth and aquatic lifestyle," Nature Communications, Nature, vol. 5(1), pages 1-13, May.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. David Wickell & Li-Yaung Kuo & Hsiao-Pei Yang & Amra Dhabalia Ashok & Iker Irisarri & Armin Dadras & Sophie de Vries & Jan de Vries & Yao-Moan Huang & Zheng Li & Michael S. Barker & Nolan T. Hartwick , 2021. "Underwater CAM photosynthesis elucidated by Isoetes genome," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    2. Liang Ma & Ke-Wei Liu & Zhen Li & Yu-Yun Hsiao & Yiying Qi & Tao Fu & Guang-Da Tang & Diyang Zhang & Wei-Hong Sun & Ding-Kun Liu & Yuanyuan Li & Gui-Zhen Chen & Xue-Die Liu & Xing-Yu Liao & Yu-Ting Ji, 2023. "Diploid and tetraploid genomes of Acorus and the evolution of monocots," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Jessen V. Bredeson & Jessica B. Lyons & Ibukun O. Oniyinde & Nneka R. Okereke & Olufisayo Kolade & Ikenna Nnabue & Christian O. Nwadili & Eva Hřibová & Matthew Parker & Jeremiah Nwogha & Shengqiang Sh, 2022. "Chromosome evolution and the genetic basis of agronomically important traits in greater yam," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:52:y:2016:i:3:id:134-2015-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.