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Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine

Author

Listed:
  • Qin ZHANG

    (College of Landscape Architecture, Beijing Forestry University, Beijing, P.R. China
    College of Landscape and Travel and)

  • Fan ZHANG

    (College of Landscape and Travel and)

  • Baohui LI

    (College of Forestry, Agricultural University of Hebei, Baoding, P.R. China)

  • Luosha ZHANG

    (College of Forestry, Agricultural University of Hebei, Baoding, P.R. China)

  • Huiqin SHI

    (College of Landscape and Travel and)

Abstract

Trollius chinensis Bunge is one of the important ornamental and medicinal plants cultivated in China. In this study, the tetraploid plants of Trollius chinensis were successfully induced by colchicine and their morphological, cytological and fertility characteristics were investigated. The results indicated that the tetraploid species of Trollius chinensis (2n = 4x = 32) was efficiently induced from the diploid species (2n = 2x = 16) by colchicine under 0.05% to 0.2% concentrations, with the best induction under treatment combinations of 0.1%, 24h duration and 0.2%, 12h duration. In comparison with the diploid plants, the tetraploid plants exhibited dramatically varied phenotypic features, showing increased leaf length and width, petiole, flower and pollen diameters. In addition, they also exhibited increased stomatal size and chloroplast numbers in guard cells. Tetraploidy reduced the pollen fertility. Nevertheless, the maintenance of female fertility makes the tetraploid a potential germplasm for further polyploid breeding. Our investigation provides a potential design for an effective pathway to generate varieties of this plant species with chromosome doubling.

Suggested Citation

  • Qin ZHANG & Fan ZHANG & Baohui LI & Luosha ZHANG & Huiqin SHI, 2016. "Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 52(1), pages 34-38.
    • Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:89-2015-cjgpb
    DOI: 10.17221/89/2015-CJGPB
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