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Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes

Author

Listed:
  • Chao-Wen SHE

    (Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua, Hunan, P.R. China
    Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua University, Huaihua, Hunan, P.R. China
    Department of Life Sciences, Huaihua University, Huaihua, Hunan, P.R. China)

  • Xiang-Hui JIANG

    (Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua, Hunan, P.R. China
    Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua University, Huaihua, Hunan, P.R. China
    Department of Life Sciences, Huaihua University, Huaihua, Hunan, P.R. China)

Abstract

The mitotic chromosomes of Lablab purpureus (L.) Sweet were characterised using sequential combined propidium iodide (PI) and 4',6-diamidino-2-phenylindole (DAPI) (CPD) staining and fluorescence in situ hybridisation (FISH) with 5S and 45S rDNA probes. The detailed karyotype of this species was established using prometaphase chromosomes. After CPD staining, CPD and DAPI+ bands were shown simultaneously. CPD bands occurred in the proximal regions of the long arms of all chromosome pairs and at all 45S rDNA sites, while the DAPI+ bands appeared in all centromeres. FISH with rDNA probes revealed one 5S locus and eight 45S loci. The single 5S locus was interstitially located on the long arms of the shortest chromosome pair. Among the 45S loci, two large loci were located in the secondary constrictions of the short arms of two chromosome pairs; six small or minimal loci were proximally located on the short or long arms of six chromosome pairs. Each prometaphase chromosome pair could be identified using the CPD and DAPI+ bands, the rDNA-FISH signals in combination with the chromosome measurements and condensation patterns. The karyotype was formulated as 2n = 2x = 22 = 14m (2SAT) + 6sm + 2st (2SAT), and the asymmetry indices, CI, A1, A2, As K%, AI and the Stebbins category were 38.23 ± 7.06, 0.36, 0.31, 61.99, 5.68 and 2B, respectively.

Suggested Citation

  • Chao-Wen SHE & Xiang-Hui JIANG, 2015. "Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 51(3), pages 110-116.
    • Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:3:id:32-2015-cjgpb
    DOI: 10.17221/32/2015-CJGPB
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    References listed on IDEAS

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    1. Ali, M. & Hasan, M. & Ahmad, Q., 2011. "Karyotype analysis in lignosus bean (Dipogon lignosus) and lablab bean (Lablab purpureus)," Journal of the Bangladesh Agricultural University, Bangladesh Agricultural University Research System (BAURES), vol. 9.
    2. Ali, M.A. & Hasan, M.M & Mia, M.S & Ahmad, Q.N, 2011. "Karyotype analysis in lignosus bean (Dipogon lignosus) and lablab bean (Lablab purpureus)," Journal of the Bangladesh Agricultural University, Bangladesh Agricultural University Research System (BAURES), vol. 9.
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