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Microsatellite analysis indicates the specific genetic basis of Czech bolting garlic

Author

Listed:
  • Jaroslava OVESNÁ

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Leona LEIŠOVÁ-SVOBODOVÁ

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Ladislav KUČERA

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

Abstract

Garlic, Allium sativum L., is a vegetable long used for culinary and medical purposes. A certain level of garlic quality is required by the local consumers, which is usually preserved by the varieties grown in that region. The aim was to establish an assay offering fast and inexpensive differentiation of garlic varieties. Length polymorphism of microsatellite loci (SSR, ILP markers) is often used in such a case. No assays have been described earlier. A set of SSR and newly used ILP markers has been assembled and verified. SSR loci ASM53, ASM072, ASA08 and ASA17 were the most polymorphic. Up to 18 alleles were scored per these loci. Monomorphic loci were identified, and excluded from the assay. The assay allows for the authenticity and confirmation of Czech garlic varieties. Moreover, a cluster analysis separated the Czech bolting varieties, indicating their specific genetic basis. The breeding potential of contemporary garlic varieties and lines is discussed.

Suggested Citation

  • Jaroslava OVESNÁ & Leona LEIŠOVÁ-SVOBODOVÁ & Ladislav KUČERA, 2014. "Microsatellite analysis indicates the specific genetic basis of Czech bolting garlic," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 50(3), pages 226-234.
    • Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:3:id:82-2014-cjgpb
    DOI: 10.17221/82/2014-CJGPB
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    References listed on IDEAS

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    1. Jana Horníčková & Roman Kubec & Karel Cejpek & Jan Velíšek & Jaroslava Ovesná & Helena Stavělíková, 2010. "Profiles of S-alk(en)ylcysteine sulfoxides in various garlic genotypes," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 28(4), pages 298-308.
    2. Jana Horníčková & Roman Kubec & Jan Velíšek & Karel Cejpek & Jaroslava Ovesná & Helena Stavělíková, 2011. "Changes of S-alk(en)ylcysteine sulfoxide levels during the growth of different garlic morphotypes," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(4), pages 373-381.
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    Cited by:

    1. Pavel Svoboda & Jakub Vašek & Pavel Vejl & Jaroslava Ovesná, 2020. "Genetic features of Czech blue poppy (Papaver somniferum L.) revealed by DNA polymorphism," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 38(3), pages 198-202.

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