IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v50y2014i3id200-2013-cjgpb.html
   My bibliography  Save this article

Green tissue-specific analysis of a cloned rbcS promoter from Lemna gibba

Author

Listed:
  • Youru WANG

    (Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Huangshi, Hubei, P.R. China
    Hubei Key Laboratory of Edible Wild Plant Conservation & Utilization, Huangshi, Hubei, P.R. China
    Life Science College, Hubei Normal University, Huangshi, Hubei, P.R. China)

Abstract

Many plant genetic engineering taskss require the spatial expression of genes which in turn depends upon the availability of specific promoters. The present paper analyses the green-tissue characteristics of a new L. gibba rbcS promoter driving the expression of the gus gene in transgenic tobacco. A 1491 bp rbcS (small subunit of ribulose bisphosphate carboxylase) promoter was isolated from Lemna gibba. The sequence analysis revealed that this promoter is different from the previously reported rbcS promoter and is named SSU5C. A 1438 bp fragment of the SSU5C promoter was fused with the gus gene and transgenic tobacco plants were generated. The analysis of T1 tobacco p1438-gus revealed that GUS expression driven by the SSU5C promoter was detected in the green part of vegetative organs. The promoter deletion analysis confirmed a region from position -152 to -49 relative to the start of transcription containing boxes X, Y and Z, while a positive regulatory region conferred green tissue-specific expression. Further functional analysis of constructs of box-X, Y, Z, which was fused with the basal SSU5C promoter, confirmed that the boxes X, Y and Z represent the new minimized functional promoter, respectively, and are able to direct green tissue-specific expression. This promoter may be used for gene expression in a tissue-specific manner in plant molecular breeding.

Suggested Citation

  • Youru WANG, 2014. "Green tissue-specific analysis of a cloned rbcS promoter from Lemna gibba," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 50(3), pages 235-240.
    • Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:3:id:200-2013-cjgpb
    DOI: 10.17221/200/2013-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/200/2013-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/200/2013-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/200/2013-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Keywords

    Lemna gibba; rbcS; SSU5C promoter; tissue characterization;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:50:y:2014:i:3:id:200-2013-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.