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Identification of an active 1Ay gene from Triticum turgidum ssp. dicoccoides

Author

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  • Zhe-Guang BI

    (Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
    Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education/Sichuan Agricultural University, Ya'an, Sichuan, P.R. China)

  • Bi-Hua WU

    (Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
    Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education/Sichuan Agricultural University, Ya'an, Sichuan, P.R. China)

  • Xi-Gui HU

    (Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
    Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education/Sichuan Agricultural University, Ya'an, Sichuan, P.R. China)

  • Xiao-Hui GUO

    (Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
    Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education/Sichuan Agricultural University, Ya'an, Sichuan, P.R. China)

  • Deng-Cai LIU

    (Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
    Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education/Sichuan Agricultural University, Ya'an, Sichuan, P.R. China)

  • You-Liang ZHENG

    (Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
    Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education/Sichuan Agricultural University, Ya'an, Sichuan, P.R. China)

Abstract

The high molecular weight glutenin subunit (HMW-GS), encoded by the 1Ay gene, unexpressed in common wheat, exists in diploid and tetraploid wheats. An active 1Ay gene was first cloned from wild emmer wheat (T. turgidum ssp. dicoccoides, 2n = 4x = 28, AABB), the oldest species in emmer wheat. Here, a novel subunit encoded by the 1Ay gene (JF519636) present in T. turgidum ssp. dicoccoides line D141 was characterized. The protein had 608 amino acids with six cysteine residues and showed faster electrophoretic mobility than 1Dy12 from common wheat. Compared with previously reported 1Ay subunits, it contained 16 single point mutations (SPMs). Comparative and phylogenetic sequence analyses suggested that this gene was more similar to the 1Ay gene from the diploid species (2n = 2x = 14, AA) T. urartu than from T. monococcum ssp. aegilopoides. Its predicted secondary protein structure possessed a different content of motifs relative to the 1Ay gene (AY245578) from T. urartu, which had similar electrophoretic mobility. In the central repetitive domain, JF519636 had more β-turns and β-bends than the 1Ay subunit AY245578. These structural characteristics in JF519636 could possibly be associated with specific gluten properties.

Suggested Citation

  • Zhe-Guang BI & Bi-Hua WU & Xi-Gui HU & Xiao-Hui GUO & Deng-Cai LIU & You-Liang ZHENG, 2014. "Identification of an active 1Ay gene from Triticum turgidum ssp. dicoccoides," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 50(3), pages 208-215.
    • Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:3:id:195-2012-cjgpb
    DOI: 10.17221/195/2012-CJGPB
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