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Detection of genetic relationships among spring and winter triticale (× Triticosecale Witt.) and rye cultivars (Secale cereale L.) by using retrotransposon-based markers

Author

Listed:
  • Andrej Trebichalský

    (Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)

  • Ruslan Kalendar

    (Institute of Biotechnology, MTT/BI Plant Genomics Lab, University of Helsinki, Helsinki, Finland)

  • Alan Schulman

    (Institute of Biotechnology, MTT/BI Plant Genomics Lab, University of Helsinki, Helsinki, Finland)

  • Olga Stratula

    (Plant Breeding and Genetics Institute, Odessa, Ukraine)

  • Zdenka Gálová

    (Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic
    Centre of Excellence for White-green Biotechnology, Chemical Institute SAV, Nitra, Slovak Republic)

  • Želmíra Balážova

    (Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)

  • Milan Chňapek

    (Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)

Abstract

In the present research, we aimed to detect and evaluate the level of long terminal repeat (LTR) retrotransposons (WIS2, Wilma, Daniela, and Wham) intraspecific variability and intron polymorphism for β-amylase (BAMY) genes in 37 winter and 25 spring triticale cultivars coming from European countries and the USA and 5 Finnish rye cultivars. The triticale and rye genotypes differ significantly with respect to the patterns of the four explored LTR retrotransposons. A neighbour-joining dendrogram has separated all triticale and rye cultivars into three principal clusters: all winter triticale, all spring triticale and all rye cultivars. We have proved that retrotransposon-based markers can be used for differentiation of triticale and rye cultivars.

Suggested Citation

  • Andrej Trebichalský & Ruslan Kalendar & Alan Schulman & Olga Stratula & Zdenka Gálová & Želmíra Balážova & Milan Chňapek, 2013. "Detection of genetic relationships among spring and winter triticale (× Triticosecale Witt.) and rye cultivars (Secale cereale L.) by using retrotransposon-based markers," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 49(4), pages 171-174.
    • Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:4:id:56-2013-cjgpb
    DOI: 10.17221/56/2013-CJGPB
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