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Integration and characterization of T-DNA insertion in upland cotton

Author

Listed:
  • Xiaojie YANG

    (State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
    Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, P.R. China)

  • Fuguang LI

    (State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China)

  • Xueyan ZHANG

    (State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China)

  • Kun LIU

    (State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China)

  • Qianhua WANG

    (State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China)

  • Chaojun ZHANG

    (State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China)

  • Chuanliang LIU

    (State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China)

  • Wei ZHU

    (Agronomy College of Henan Agricultural University, Zhengzhou, P.R. China)

  • Guofang SHAN

    (State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China)

  • Chee-Kok CHIN

    (Department of Plant Science, Rutgers University, New Jersey, USA)

  • Weiping FANG

    (Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, P.R. China)

Abstract

Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integration of one or two T-DNA copies, 54% had three or more copies. Among 63 amplified products at LB junctions, 51% showed co-transformation of the vector backbone, 30% retained a portion of LB ranging from 3 to 23 bp, and 19% showed deletions ranging from 1 to 148 bp from the LB inner end. In contrast, all of the cleavage sites were located in the inner region of RB. The distribution of T-DNA insertions in upland cotton genome included coding sequences, transposons, plastid-derived sequences and microsatellites.

Suggested Citation

  • Xiaojie YANG & Fuguang LI & Xueyan ZHANG & Kun LIU & Qianhua WANG & Chaojun ZHANG & Chuanliang LIU & Wei ZHU & Guofang SHAN & Chee-Kok CHIN & Weiping FANG, 2013. "Integration and characterization of T-DNA insertion in upland cotton," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 49(2), pages 51-57.
    • Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:217-2012-cjgpb
    DOI: 10.17221/217/2012-CJGPB
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