IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v48y2012i4id111-2012-cjgpb.html
   My bibliography  Save this article

Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.)

Author

Listed:
  • Daniel Mihálik

    (Plant Production Research Center Piešťany, Piešťany, Slovak Republic)

  • Edita Gregová

    (Plant Production Research Center Piešťany, Piešťany, Slovak Republic)

  • Petr Galuszka

    (Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic)

  • Ludmila Ohnoutková

    (Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic)

  • Tatiana Klempová

    (Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak Technical University, Bratislava, Slovak Republic)

  • Katarína Ondreičková

    (Plant Production Research Center Piešťany, Piešťany, Slovak Republic)

  • Marcela Gubišová

    (Plant Production Research Center Piešťany, Piešťany, Slovak Republic
    Department of Botany and Genetics, Faculty of Natural Sciences, Constantine The Philosopher University in Nitra, Nitra, Slovak Republic)

  • Jozef Gubiš

    (Plant Production Research Center Piešťany, Piešťany, Slovak Republic)

  • Ján Kraic

    (Plant Production Research Center Piešťany, Piešťany, Slovak Republic)

Abstract

A novel high-molecular-weight glutenin subunit encoded by the Glu-1D locus was identified in hexaploid wheat (Triticum aestivum L.) cultivar Noe and was designated as 1Dy12.3. This subunit differed in SDS-PAGE mobility from the well-known 1Dy10 and 1Dy12 subunits that are also encoded by this locus. An analysis of the gene sequences confirmed the uniqueness of 1Dy12.3 and revealed that it is most closely related to the 1Dy12 subunit. The size of the deduced protein was calculated to be 67 884 Da, which is different from the 1Dy10 and 1Dy12 subunits (67 475 Da and 68 713 Da, respectively). The 1Dy12.3 protein consists of 652 residues, with a highly conserved signal sequence and N- and C-terminal domains, although the central repetitive domain comprising motifs of hexapeptide (PGQGQQ) and nonapeptide (GYYPTSLQQ) repeats was less conserved. The 1Dy12.3 subunit demonstrates fewer QHPEQG hexapeptide motifs and exhibits an increased number of methionine residues in comparison to the other characterised high-molecular-weight glutenin subunits. The 1Dy12.3 subunit was cloned and expressed in Escherichia coli and was detected with a prolamin-specific antibody. The size of the detected immunocomplex corresponded to the native 1Dy12.3 protein isolated from grains. The existence and characterisation of this novel high-molecular-weight glutenin subunit increases the diversity of the glutenins encoded by the Glu-1D locus.

Suggested Citation

  • Daniel Mihálik & Edita Gregová & Petr Galuszka & Ludmila Ohnoutková & Tatiana Klempová & Katarína Ondreičková & Marcela Gubišová & Jozef Gubiš & Ján Kraic, 2012. "Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.)," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 48(4), pages 157-168.
    • Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:111-2012-cjgpb
    DOI: 10.17221/111/2012-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/111/2012-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/111/2012-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/111/2012-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. L. Dotlačil & J. Hermuth & Z. Stehno & V. Dvořáček & J. Bradová & L. Leišová, 2010. "How can wheat landraces contribute to present breeding?," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 46(SpecialIs), pages 70-74.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. V. Dvořáček & L. Dotlačil & J. Hermuth & A. Prohasková & Z. Stehno & L. Svobodová, 2011. "The utilization of wheat genetic resources in breeding for bread-making quality," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 47(SpecialIs), pages 71-76.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:111-2012-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.