IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v47y2011i4id140-2011-cjgpb.html
   My bibliography  Save this article

Identification of apple scab and powdery mildew resistance genes in Czech apple (Malus × domestica) genetic resources by PCR molecular markers

Author

Listed:
  • Josef Patzak

    (Hop Research Institute, Žatec, Czech Republic)

  • František Paprštein

    (Research and Breeding Institute of Pomology in Holovousy, Holovousy, Hořice v Podkrkonoší, Czech Republic)

  • Alena Henychová

    (Hop Research Institute, Žatec, Czech Republic)

Abstract

The presence of genes for resistance to scab (Venturia inaequalis) and powdery mildew (Podosphaera leucotricha) was studied using molecular markers in a sample of 279 apple cultivars from the Czech collection of apple genetic resources. The sample comprised 37 cultivars supposed to have the Vf gene for scab resistance, 97 reference world cultivars and 145 old and local cultivars. Six PCR molecular markers for the scab resistance genes Vf, Vm, Vbj, Vr and Vh and three PCR molecular markers for the powdery mildew resistance genes Pl-w, Pl-1 and Pl-d were used. The marker for the major scab resistance gene Vf was detected in all cultivars supposed to have Vf, except in Romus 1, and in the three small-fruited cultivars Malus Evereste, Golden Gem and Hilleri. The markers of the Vr and Vh scab resistance genes were detected in 22 cultivars in combination with the marker for Vf, in 56 reference world cultivars and in 82 old and local apple cultivars. PCR molecular markers for one or two of the powdery mildew resistance genes were detected in the small-fruited cultivars Malus Evereste, Golden Gem, prof. Sprengeri and Hilleri; and in the larger fruited cultivars Hagloe Crab, Borovinka and Tita Zetei. We did not find markers for the scab resistance genes Vm and Vbj in any of the studied cultivars. They are absent also in the remaining part of the Czech collection of apple genetic resources. PCR molecular markers are useful tools for the identification of resistance genes within apple germplasm collections and can be used to increase the number of sources for disease resistance in breeding programmes.

Suggested Citation

  • Josef Patzak & František Paprštein & Alena Henychová, 2011. "Identification of apple scab and powdery mildew resistance genes in Czech apple (Malus × domestica) genetic resources by PCR molecular markers," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 47(4), pages 156-165.
    • Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:140-2011-cjgpb
    DOI: 10.17221/140/2011-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/140/2011-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/140/2011-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/140/2011-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:140-2011-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.