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Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

Author

Listed:
  • Tian Li

    (Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China)

  • Jing Kuan Sun

    (Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China)

  • Zhao Hua Lu

    (Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China)

  • Qing Liu

    (Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China)

Abstract

The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium-mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T-DNA) in Agrobacterium, plant reporter gene gus, and plant selection marker gene hpt. The recombinant binary vector pBRSAg was transformed into Agrobacterium tumefaciens strains by using the freeze-thaw method. Tomato cotyledon explants were transformed by A. tumefaciens and plants were regenerated on selection medium. GUS staining, PCR and PCR-Southern analysis of the plants were positive. It was for the first time shown that the HBsAg (hepatitis B surface antigen) gene was introduced into tomato plants. The expression of transgene is under investigation.

Suggested Citation

  • Tian Li & Jing Kuan Sun & Zhao Hua Lu & Qing Liu, 2011. "Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 47(2), pages 69-77.
    • Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:2:id:5-2011-cjgpb
    DOI: 10.17221/5/2011-CJGPB
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