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Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley

Author

Listed:
  • Ludmila Holková

    (Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic)

  • Pavlína Mikulková

    (Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic)

  • Pavlína Hrstková

    (Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic)

  • Ilja Tom Prášil

    (Department of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Marta Bradáčová

    (Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic)

  • Pavla Prášilová

    (Department of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Oldřich Chloupek

    (Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic)

Abstract

The sequences of the closely linked barley dehydrin genes Dhn4 and Dhn7 are both known to vary allelically. Here we associated allelic constitution at Dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of Dhn4 and a 30 bp deletion in exon1 of Dhn7 was restricted to six-rowed winter and intermediate varieties characterised by relatively higher frost tolerance (12 genotypes; LT50 from -14.2°C to -15.6°C). The alternative combination was present with one exception (sixrowed winter variety Alissa) only in spring and two-rowed winter varieties (17 genotypes; LT50 from -10.0°C to -14.3°C). The genetic linkage between Dhn4 and Dhn7 identified e.g. in Dictoo and Morex varieties was verified by segregation analysis of F2 plants from a cross between two genotypes carrying different allelic combination of Dhn4 and Dhn7 genes (two-rowed spring variety Akcent × six-rowed winter variety Okal). The potential of the former allelic combination as a marker for enhanced frost tolerance was tested in a sample of F5 derivatives of a cross between the two-rowed winter type variety Monaco (Akcent allele combination) and the six-rowed winter type variety Okal. Plants with the Okal allele combination showed significantly higher frost tolerance than those with the alternative growth habit. The effect of ear type on frost tolerance was insignificant.

Suggested Citation

  • Ludmila Holková & Pavlína Mikulková & Pavlína Hrstková & Ilja Tom Prášil & Marta Bradáčová & Pavla Prášilová & Oldřich Chloupek, 2010. "Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 46(4), pages 149-158.
    • Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:90-2010-cjgpb
    DOI: 10.17221/90/2010-CJGPB
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    Cited by:

    1. I.T. Prášil & J. Chrpová & Z. Stehno, 2011. "Sixty years of the Crop Research Institute and its impact on plant breeding," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 47(4), pages 172-180.

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