IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v43y2007i4id2074-cjgpb.html
   My bibliography  Save this article

Selection of DNA markers for detection of extreme resistance to potato virus Y in tetraploid potato (Solanum tuberosum L.) F1 progenies

Author

Listed:
  • Ján Heldák

    (Potato Research and Breeding Institute, Veľ ká Lomnica, Slovak Republic)

  • Milan Bežo

    (Department of Genetics and Plant Breeding, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)

  • Veronika Štefúnová

    (Department of Genetics and Plant Breeding, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)

  • Andrea Galliková

    (Potato Research and Breeding Institute, Veľ ká Lomnica, Slovak Republic)

Abstract

Marker-assisted selection is progressively introduced into potato breeding especially in the case of monogenically inherited traits. Varieties and clones with extreme resistance to potato virus Y (PVY) from German, Hungarian, Polish, Dutch and Slovak breeding programmes were used as female parents in 6 crosses. F1 progenies were subjected to the bulk segregant analysis. Polymerase chain reaction (PCR) based markers for Rystoand Ryadg viral resistance alleles were used for the evaluation of varieties, clones and seedlings of F1 progenies. All resistant F1 genotypes were reliably identified using three molecular markers, STM0003, GP122718 and GP122406, associated with the Rysto allele. No genotype corresponded with phenotypic data and Ryadg markers. Ryadg and three selected Rysto markers were applied to 40 genotypes from an in vitro gene bank and 14 genotypes were recognized to have Rysto and none of them possessed Ryadg Indirect selection is based on DNA polymorphism linked to the extreme resistance to PVY resulting in error when polymorphism is not conserved in all genetic backgrounds. A failure to detect extreme resistance to PVY using the Rysto markers in variety Santé, resistant progeny of variety Santé and clone Y01-30 may be linked to recombination events or differentSolanum stoloniferum background.

Suggested Citation

  • Ján Heldák & Milan Bežo & Veronika Štefúnová & Andrea Galliková, 2007. "Selection of DNA markers for detection of extreme resistance to potato virus Y in tetraploid potato (Solanum tuberosum L.) F1 progenies," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 43(4), pages 125-134.
    • Handle: RePEc:caa:jnlcjg:v:43:y:2007:i:4:id:2074-cjgpb
    DOI: 10.17221/2074-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/2074-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/2074-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/2074-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:43:y:2007:i:4:id:2074-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.