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Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding

Author

Listed:
  • Marie Špunarová

    (Agrotest fyto, Ltd., Kroměříž, Czech Republic)

  • Milan Pouch

    (Agrotest fyto, Ltd., Kroměříž, Czech Republic)

  • Jaroslav Špunar

    (Agrotest fyto, Ltd., Kroměříž, Czech Republic)

Abstract

The success in hybridization of two-rowed spring barley genotypes of various origins was verified using the codominant SSR (Simple Sequence Repeat) marker HVWAXY which is located in the coding sequence of the waxy gene. The primer pair F 5' AAG ACG TGG TGT TCG TGT G 3' and R 5' ATG GTT CCA GGG GTA AGT TC 3' generated the PCR product of approximately 200 bp in the varieties/breeding lines Maridol, Bojos, Malz, Xanadu, Isotta, Josefin, Native, Sebastian, Conrad, KM 2436, KM 2439, KM 2629, KM-H-1320, whereas the product of about 250 bp was amplified in the lines KM 2416 and Br 7571h33. The parental genotypes possessing different alleles of the marker were crossed and the segregating lines of the F3 generation were characterized employing molecular methods (marker-assisted selection). This prescreening enabled to work with a lower number of individuals (selfed individuals were excluded) at the initial stages of the breeding process leading to the acceleration of selection as a particular phase of breeding.

Suggested Citation

  • Marie Špunarová & Milan Pouch & Jaroslav Špunar, 2007. "Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 43(4), pages 144-148.
    • Handle: RePEc:caa:jnlcjg:v:43:y:2007:i:4:id:1900-cjgpb
    DOI: 10.17221/1900-CJGPB
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    References listed on IDEAS

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    1. Brian J. Steffenson & Kevin P. Smith, 2006. "Breeding Barley for Multiple Disease Resistance in the Upper MidwestRegion of the USA," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 42(3), pages 79-86.
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