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Assessment of genetic diversity of yellow-seeded rapeseed (Brassica napus L.) accessions by AFLP markers

Author

Listed:
  • Chengyu YU

    (College of Agronomy, Northwest A&F University, Yangling, China)

  • Leona Leišová

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Vratislav Kučera

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Miroslava Vyvadilová

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Jaroslava Ovesná

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Ladislav Dotlačil

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Shengwu HU

    (College of Agronomy, Northwest A&F University, Yangling, China)

Abstract

The genetic diversity of 35 yellow-seeded Brassica napus L. accessions originating from China, Czech Republic and Poland was assessed by means of Amplified Fragment Length Polymorphism (AFLP) markers based on multiplex PCR using multi-colour fluorescent-labelled primers. Five brown-seeded accessions originating from China and France were selected as outliers. In total, 632 peaks were generated by AFLP reaction using 18 primer combinations. Only distinctly polymorphic markers among them were scored. In total, 242 polymorphic markers were detected with an average of 13.4 markers per primer combination. The AFLP analysis separated forty studied accessions into Chinese and European groups by UPGMA clustering and Principal Coordinates Analysis (PCA). The grouping of accessions based on the cluster analysis and PCA was generally consistent with known pedigree information and geographic origin. Notable geographical divergence was found between Chinese and European yellow-seeded accessions. This information is useful for yellow-seeded hybrid breeding and encouraging breeders to exchange their germplasm as to enlarge the genetic diversity of breeding accessions.

Suggested Citation

  • Chengyu YU & Leona Leišová & Vratislav Kučera & Miroslava Vyvadilová & Jaroslava Ovesná & Ladislav Dotlačil & Shengwu HU, 2007. "Assessment of genetic diversity of yellow-seeded rapeseed (Brassica napus L.) accessions by AFLP markers," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 43(3), pages 105-112.
    • Handle: RePEc:caa:jnlcjg:v:43:y:2007:i:3:id:2071-cjgpb
    DOI: 10.17221/2071-CJGPB
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    References listed on IDEAS

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    1. S.W. HU & J. Ovesná & L. Kučera & V. Kučera & M. Vyvadilová, 2003. "Evaluation of genetic diversity of Brassica napus germplasm from China and Europe assessed by RAPD markers," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 49(3), pages 106-113.
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    Cited by:

    1. Lenka HAVLÍČKOVÁ & Eva JOZOVÁ & Andrea RYCHLÁ & Miroslav KLÍMA & Vratislav KUČERA & Vladislav ČURN, 2014. "Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 50(3), pages 216-225.

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